Publication:
Glycaemic response in pigs derived from assisted reproductive technologies: a long-term follow-up study

Simple item page
dc.contributor.authorRomar, Raquel
dc.contributor.authorRomero-Aguirregomezcorta, Jon
dc.contributor.authorHeras, Sonia
dc.contributor.authorParis-Oller, Evelyne
dc.contributor.authorSoriano-Ubeda, Cristina
dc.contributor.authorGadea, Joaquin
dc.contributor.authorCoy, Pilar
dc.contributor.authorCanovas, Sebastian
dc.contributor.departmentFisiologíaes
dc.date.accessioned2025-09-05T09:54:14Z
dc.date.available2025-09-05T09:54:14Z
dc.date.copyright© 2024
dc.date.issued2024
dc.description.abstractEmerging evidence suggests that assisted reproductive technologies (ART) may predispose individuals to an increased risk of metabolic disorders including diabetes. Previous studies have reported a different glycaemic response in growing pigs derived from ART, but it is unknown whether these differences persist into adulthood and old age. The aim of the present work was to gain insight into the evolution of glycaemic response of ART-derived pigs across their life. Pigs born through artificial insemination (AI group) and transfer of in vitro-produced embryos (IVP group) underwent a glucose challenge at young age (45 days), adulthood (365 days), and old age (1250 days). The number of animals, respectively for AI and IVP groups, were 16 and 29 (young), 13 and 21 (adult), and 9 and 13 (old). Oral glucose tolerance test (OGTT; 1.75g/kg body weight) was performed after overnight fasting and 1h water withdrawn. Blood samples were obtained from auricular vein prior (T=0 min) and 15, 30, 45, 60, 90, 120, 150 min following glucose intake. Glycemia was immediately determined by glucometer test strips (GlucoMenLX Plus). The area under the curve analysis was used to identify differences (p < 0.05) between age and experimental groups. Minimum and maximum glycemia recorded during the three OGTT tests were 22.0 and 319.0 mg/dl respectively. Both the origin of the animals (AI vs. IVP) and the age (young-adult-old) significantly affected the glycaemic response (p < 0.05). At a similar age, there were no differences in fasting glucose between AI and IVP groups (young: 81.70±4.67 and 90.25±8.27 mg/dl; adult: 91.13±7.41 and 87.70±7.14 mg/dl; old: 65.12±3.50 and 59.93±4.12 mg/dl) being all data withing the physiological range for pigs (60.00-100.00 mg/dl). Mean glycaemia during OGTT was 25-30 mg/dl higher in young and adult animals compared to old pigs (p < 0.05).Evolution of glycemia curves showed a monophasic pattern during youth in bothgroups, as it is commonly observed in young pigs and we have previously described. Interestingly, the response pattern shifted into a biphasic curve in adult-AI pigs, returning to a monophasic response during old age, whereas in IVP animals the biphasic response appeared only during old age. The lack of studies in older pigs makes difficult to interpret the biphasic response which in other species is associated with a certain resistance to insulin. Moreover, differences between origin of animals were observed at young and old ages (p < 0.05). Therefore, young-IVP animals had higher AUC during the initial response to glucose challenge (15, 30 and 45 min) than young-AI pigs. However, old-IVP had lower AUC than old-AI pigs at the intermediate time of 60 min due to the biphasic glucose peak around this time. No differences between groups were observed during adulthood. In conclusion, naturally and artificially conceived pigs show different responses to glucose challenge across life. The clinical relevance and implications of such differences should be further studied.es
dc.formatapplication/pdfes
dc.format.extent2es
dc.identifier.eisbnABSTRACT book --57th Society for Study of Reproduction (SSR) Annual Conferencees
dc.identifier.urihttp://hdl.handle.net/10201/158200
dc.languageenges
dc.publisherSociety for Study of Reproduction (SSR)es
dc.relationThis study is part of project PID2020113366RB-I00 funded by MCIN/AEI/10.13039/501100011033/ and “FEDER Una manera de hacer Europa”.es
dc.relation.ispartof57th Society for Study of Reproduction (SSR) Annual Conferencees
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectGlycaemiaes
dc.subjectPigses
dc.subjectAssisted Reproductive Technologieses
dc.subjectLong Termes
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleGlycaemic response in pigs derived from assisted reproductive technologies: a long-term follow-up studyes
dc.typeinfo:eu-repo/semantics/conferenceObjectes
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2024_SSR Romar et al.pdf
Size:
87.32 KB
Format:
Adobe Portable Document Format
Description:
2024_SSR Romar et al
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.26 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Ponencias y Comunicaciones