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Histological parameters of the adrenal cortex after testosterone application in a rat model of the andropause

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dc.contributor.authorAjdžanović, Vladimir Z.
dc.contributor.authorJarić, Ivana M.
dc.contributor.authorŽivanović, Jasmina B.
dc.contributor.authorFilipović, Branko R.
dc.contributor.authorŠošić Jurjević, Branka T.
dc.contributor.authorRistić, Nataša M.
dc.contributor.authorStanković, Sanja D.
dc.date.accessioned2021-11-16T09:32:50Z
dc.date.available2021-11-16T09:32:50Z
dc.date.issued2016
dc.description.abstractHistological analysis of the adrenal cortex, after testosterone application in a rat model of the andropause, was the main subject of the present study. Middle-aged Wistar rats were divided into shamoperated (SO; n=8), orchidectomized (Orx; n=8) and testosterone treated orchidectomized (Orx+T; n=8) groups. Testosterone propionate (5 mg/kg b.m. /day) was administered for three weeks, while SO and Orx groups received the vehicle alone. Histological objectives were achieved using stereology, histochemistry and steroid receptor immunostaining. The concentrations of testosterone, aldosterone, corticosterone and DHEA were determined by immunoassays. Expectedly, increased (p<0.05) serum concentration of testosterone was observed in Orx+T group. The volume of ZG cells and nuclei increased in Orx+T animals by 50% and 25% (p<0.05) respectively, but the serum concentrations of aldosterone decreased (p<0.05) by 60%, all compared to the same parameters in Orx group. The immunostaining for androgen receptors (ARs) suggested their cytoplasmic localization in ZG cells of Orx+T rats. Volume of the ZF cell nuclei in Orx+T group decreased (p<0.05) by 17%, which was followed by the significant (p<0.05) fall in corticosterone production and secretion, all in comparison with Orx animals. Also, nuclear immunolocalization of ARs of high optical density was observed through the ZF of Orx+T group. In Orx+T rats volume of ZR cells and nuclei, and circulating DHEA concentration increased (p<0.05) by 68%, 22% and about 6.6 times respectively, compared to Orx animals. Besides the extra-receptor actions in adrenal cortex, testosterone supposedly affects some steroidogenesisrelated gene expression, as indicated by centripetal rise in the number of nuclear ARs.es
dc.formatapplication/pdfes
dc.format.extent12es
dc.identifier.doiDOI: 10.14670/HH-11-749
dc.identifier.eisbnHistology and Histopathology, Vol.31, nº11, (2016)
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/114044
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectHistologyes
dc.subjectAdrenal cortexes
dc.subjectTestosteronees
dc.subjectRatses
dc.subjectAndropausees
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleHistological parameters of the adrenal cortex after testosterone application in a rat model of the andropausees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.31,nº 11 (2016)