Publication: Histological parameters of the adrenal cortex after testosterone application in a rat model of the andropause
Authors
Ajdžanović, Vladimir Z. ; Jarić, Ivana M. ; Živanović, Jasmina B. ; Filipović, Branko R. ; Šošić Jurjević, Branka T. ; Ristić, Nataša M. ; Stanković, Sanja D.
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-749
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info:eu-repo/semantics/article
Description
Abstract
Histological analysis of the adrenal cortex,
after testosterone application in a rat model of the
andropause, was the main subject of the present study.
Middle-aged Wistar rats were divided into shamoperated (SO; n=8), orchidectomized (Orx; n=8) and
testosterone treated orchidectomized (Orx+T; n=8)
groups. Testosterone propionate (5 mg/kg b.m. /day) was
administered for three weeks, while SO and Orx groups
received the vehicle alone. Histological objectives were
achieved using stereology, histochemistry and steroid
receptor immunostaining. The concentrations of
testosterone, aldosterone, corticosterone and DHEA
were determined by immunoassays. Expectedly,
increased (p<0.05) serum concentration of testosterone
was observed in Orx+T group. The volume of ZG cells
and nuclei increased in Orx+T animals by 50% and 25%
(p<0.05) respectively, but the serum concentrations of
aldosterone decreased (p<0.05) by 60%, all compared to
the same parameters in Orx group. The immunostaining
for androgen receptors (ARs) suggested their
cytoplasmic localization in ZG cells of Orx+T rats.
Volume of the ZF cell nuclei in Orx+T group decreased
(p<0.05) by 17%, which was followed by the significant
(p<0.05) fall in corticosterone production and secretion,
all in comparison with Orx animals. Also, nuclear
immunolocalization of ARs of high optical density was
observed through the ZF of Orx+T group. In Orx+T rats
volume of ZR cells and nuclei, and circulating DHEA
concentration increased (p<0.05) by 68%, 22% and
about 6.6 times respectively, compared to Orx animals.
Besides the extra-receptor actions in adrenal cortex,
testosterone supposedly affects some steroidogenesisrelated gene expression, as indicated by centripetal rise
in the number of nuclear ARs.
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