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The transcriptome of pig spermatozoa, and its role in fertility

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dc.contributor.authorÁlvarez Rodríguez, Manuel
dc.contributor.authorMartínez, Cristina
dc.contributor.authorWright, Dominic
dc.contributor.authorBarranco, Isabel
dc.contributor.authorRoca, Jordi
dc.contributor.authorRodríguez Martínez, Heriberto
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2024-09-06T07:20:38Z
dc.date.available2024-09-06T07:20:38Z
dc.date.issued2020-02-25
dc.description© 2020 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in International Journal of Molecular Sciences. To access the final edited and published work see https://doi.org/10.3390/ijms21051572
dc.description.abstractIn the study presented here we identified transcriptomic markers for fertility in the cargo of pig ejaculated spermatozoa using porcine-specific micro-arrays (GeneChip® miRNA 4.0 and GeneChip® Porcine Gene 1.0 ST). We report (i) the relative abundance of the ssc-miR-1285, miR-16, miR-4332, miR-92a, miR-671-5p, miR-4334-5p, miR-425-5p, miR-191, miR-92b-5p and miR-15b miRNAs, and (ii) the presence of 347 up-regulated and 174 down-regulated RNA transcripts in high-fertility breeding boars, based on differences of farrowing rate (FS) and litter size (LS), relative to low-fertility boars in the (Artificial Insemination) AI program. An overrepresentation analysis of the protein class (PANTHER) identified significant fold-increases for C-C chemokine binding (GO:0019957): CCR7, which activates B- and T-lymphocytes, 8-fold increase), XCR1 and CXCR4 (with ubiquitin as a natural ligand, 1.24-fold increase), cytokine receptor activity (GO:0005126): IL23R receptor of the IL23 protein, associated to JAK2 and STAT3, 3.4-fold increase), the TGF-receptor (PC00035) genes ACVR1C and ACVR2B (12-fold increase). Moreover, two micro-RNAs (miR-221 and mir-621) were down- and up-regulated, respectively, in high-fertility males. In conclusion, boars with different fertility performance possess a wide variety of differentially expressed RNA present in spermatozoa that would be attractive targets as non-invasive molecular markers for predicting fertility.es
dc.formatapplication/pdfes
dc.format.extent23es
dc.identifier.citationInt. J. Mol. Sci. 2020, 21, 1572
dc.identifier.doihttps://doi.org/10.3390/ijms21051572
dc.identifier.issnPrint: 1661-6596
dc.identifier.issnElectronic: 1422-0067
dc.identifier.urihttp://hdl.handle.net/10201/143679
dc.languageenges
dc.publisherMDPI
dc.relationThis research was funded by the Research Council FORMAS, Stockholm (Project 2017-00946 and Project 2019-00288), the Swedish Research Council (Vetenskapsrådet, VR; project 2015-05919), the MINECO and FEDER (AGL2015-69738-R) Madrid (Spain) and the Seneca Foundation (20780/PD/18) Murcia (Spain).es
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/21/5/1572es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectTranscriptomicses
dc.subjectMicroarrays
dc.subjectBioinformatics
dc.subjectSpermatozoa
dc.subjectFertility
dc.subjectPig
dc.titleThe transcriptome of pig spermatozoa, and its role in fertilityes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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