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Nestin+cells forming spheroids aggregates resembling tumorspheres in experimental ENU-induced gliomas

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dc.contributor.authorGarcía Blanco, Alvaro
dc.contributor.authorBulnes, Susana
dc.contributor.authorPomposo, Iñigo
dc.contributor.authorCarrasco, Alex
dc.contributor.authorLafuente, José Vicente
dc.date.accessioned2021-12-13T10:22:37Z
dc.date.available2021-12-13T10:22:37Z
dc.date.issued2016
dc.description.abstractNestin+cells from spheroid aggregates display typical histopathological features compatible with cell stemness. Nestin and CD133+cells found in glioblastomas, distributed frequently around aberrant vessels, are considered as potential cancer stem cells. They are possible targets for antitumoral therapy because they lead the tumorigenesis, invasiveness and angiogenesis. However, little is known about their role and presence in low-grade gliomas. The aim of this work is to localize and characterize the distribution of these cells inside tumors during the development of experimental endogenous glioma. For this study, a single dose of Ethyl-nitrosourea was injected into pregnant rats. Double immunofluorescences were performed in order to identify stem-like and differentiated cells. Low-grade gliomas display Nestin+cells distributed throughout the tumor. More malignant gliomas show, in addition to that, a perivascular location with some Nestin+cells co-expressing CD133 or VEGF, and the intratumoral spheroid aggregates of Nestin/CD133+ cells. These structures are encapsulated by welldifferentiated VEGF/GFAP+cells. Spheroid aggregates increase in size in the most malignant stages. Spheroid aggregates have morphological and phenotypic similarities to in vitro neurospheres and could be an in vivo analogue of them. These arrangements could be a reservoir of undifferentiated cells formed to escape adverse microenvironments.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.doiDOI: 10.14670/HH-11-763
dc.identifier.eisbnHistology and Histopathology, Vol.31, nº12, (2016)es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/114866
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectStem cellses
dc.subjectNestines
dc.subjectENUes
dc.subjectGliomaes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleNestin+cells forming spheroids aggregates resembling tumorspheres in experimental ENU-induced gliomases
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.31,nº 12 (2016)