Publication: Nestin+cells forming spheroids aggregates resembling tumorspheres in experimental ENU-induced gliomas
Authors
García Blanco, Alvaro ; Bulnes, Susana ; Pomposo, Iñigo ; Carrasco, Alex ; Lafuente, José Vicente
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-763
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info:eu-repo/semantics/article
Description
Abstract
Nestin+cells from spheroid aggregates
display typical histopathological features compatible
with cell stemness. Nestin and CD133+cells found in
glioblastomas, distributed frequently around aberrant
vessels, are considered as potential cancer stem cells.
They are possible targets for antitumoral therapy because
they lead the tumorigenesis, invasiveness and
angiogenesis. However, little is known about their role
and presence in low-grade gliomas. The aim of this work
is to localize and characterize the distribution of these
cells inside tumors during the development of
experimental endogenous glioma.
For this study, a single dose of Ethyl-nitrosourea was
injected into pregnant rats. Double immunofluorescences
were performed in order to identify stem-like and
differentiated cells.
Low-grade gliomas display Nestin+cells distributed
throughout the tumor. More malignant gliomas show, in
addition to that, a perivascular location with some
Nestin+cells co-expressing CD133 or VEGF, and the
intratumoral spheroid aggregates of Nestin/CD133+
cells. These structures are encapsulated by welldifferentiated VEGF/GFAP+cells. Spheroid aggregates
increase in size in the most malignant stages.
Spheroid aggregates have morphological and
phenotypic similarities to in vitro neurospheres and
could be an in vivo analogue of them. These
arrangements could be a reservoir of undifferentiated
cells formed to escape adverse microenvironments.
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