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Antifungal effects and mechanism of action of viscotoxin A3

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dc.contributor.authorGuidici Besseghini, Ana Marcela
dc.contributor.authorPoveda Larrosa, José Antonio
dc.contributor.authorMolina Gallego, María Luisa
dc.contributor.authorCanal, Laura de la
dc.contributor.authorGonzález Ros, José Manuel
dc.contributor.authorPfüller, Karola
dc.contributor.authorPfüller, Uwe
dc.contributor.authorVillalaín Boullón, José
dc.contributor.departmentBioquímica y Biología Molecular B e Inmunología
dc.date.accessioned2024-07-04T11:31:24Z
dc.date.available2024-07-04T11:31:24Z
dc.date.issued2006-01-01
dc.description©2005 The Authors Journal compilation; FEBS. This document is the Published version of a Published Work that appeared in final form in The FEBS Journal. To access the final edited and published work see https://doi.org/10.1111/j.1742-4658.2005.05042.x
dc.description.abstractViscotoxins are cationic proteins, isolated from different mistletoe species, that belong to the group of thionins, a group of basic cysteine-rich peptides of ≈ 5 kDa. They have been shown to be cytotoxic to different types of cell, including animal, bacterial and fungal. The aim of this study was to obtain information on the cell targets and the mechanism of action of viscotoxin isoform A3 (VtA3). We describe a detailed study of viscotoxin interaction with fungal-derived model membranes, its location inside spores of Fusarium solani, as well as their induced spore death. We show that VtA3 induces the appearance of ion-channel-like activity, the generation of H2O2, and an increase in cytoplasmic free Ca2+. Moreover, we show that Ca2+ is involved in VtA3-induced spore death and increased H2O2 concentration. The data presented here strongly support the notion that the antifungal activity of VtA3 is due to membrane binding and channel formation, leading to destabilization and disruption of the plasma membrane, thereby supporting a direct role for viscotoxins in the plant defence mechanism.es
dc.embargo.termsSi
dc.formatapplication/pdfes
dc.format.extent12es
dc.identifier.doihttps://doi.org/10.1111/j.1742-4658.2005.05042.x
dc.identifier.eisbnFEBS Journal 273 (2006) 72–83es
dc.identifier.issnPrint: 1742-464X
dc.identifier.issnElectronic: 1742-4658
dc.identifier.urihttp://hdl.handle.net/10201/142880
dc.languageenges
dc.publisherFederation of European Biochemical Societies [Society Publisher]
dc.relationThis work was supported by grant BMC2002-00158 from MCYT, Spain (to J.V.). M.G. is a recipient of a predoctoral fellowship from CONICET, Argentina. The financial support of AECI, Spain, is gratefully acknowledged.es
dc.relation.publisherversionhttps://febs.onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2005.05042.x
dc.rightsinfo:eu-repo/semantics/embargoedAccesses
dc.subjectAntifungales
dc.subjectCytotoxicityes
dc.subjectDefence mechanismses
dc.subjectMistletoees
dc.subjectViscotoxinses
dc.titleAntifungal effects and mechanism of action of viscotoxin A3es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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