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Chitosan/albumin coating factorial optimization of alginate/dextran sulfate cores for oral delivery of insulin

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dc.contributor.authorPessoa, Bruno
dc.contributor.authorSandri, Giuseppina
dc.contributor.authorRibeiro, António
dc.contributor.authorCollado-González, Mar
dc.contributor.departmentBiología Celular e Histología
dc.date.accessioned2025-09-25T08:15:39Z
dc.date.available2025-09-25T08:15:39Z
dc.date.copyright© 2023 by the authors
dc.date.issued2023-03-14
dc.description.abstractThe design of nanoparticle formulations composed of biopolymers, that govern the physicochemical properties of orally delivered insulin, relies on improving insulin Stability and absorption through the intestinal mucosa while protecting it from harsh conditions in the gastrointestinal (GI) tract. Chitosan/polyethylene glycol (PEG) and albumin coating of alginate/dextran sulfate hydrogel cores are presented as a ultilayer complex protecting insulin within the nanoparticle. This study aims to optimize a anoparticle formulation by assessing the relationship between design parameters and experimental data using response surface methodology through a 3-factor 3-level optimization Box–Behnken design. While the selected independent variables were the concentrations of PEG, chitosan and albumin, the dependent variables were particle Size, polydispersity index (PDI), zeta potential, and insulin release. Experimental results showed a nanoparticle size ranging from 313 to 585 nm, with PDI from 0.17 to 0.39 and zeta potential ranging from 􀀀29 to 􀀀44 mV. Insulin bioactivity was maintained in simulated GI media with over 45% cumulative release after 180 min in a simulated intestinal medium. Based on the experimental responses and according to the criteria of desirability on the experimental region’s constraints, solutions of 0.03% PEG, 0.047% chitosan and 1.20% albumin provide an optimum nanoparticle formulation for insulin oral delivery.
dc.formatapplication/pdf
dc.format.extent18
dc.identifier.citationMarine Drugs 2023, 21, 179
dc.identifier.doihttps://doi.org/10.3390/md21030179
dc.identifier.eissn1660-3397
dc.identifier.urihttp://hdl.handle.net/10201/161570
dc.languageeng
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.relationThe authors thank Fundação para a Ciência e Tecnologia of Portugal for financial support.
dc.relation.publisherversionhttps://www.mdpi.com/1660-3397/21/3/179
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBiopolymers
dc.subjectBox–Behnken
dc.subjectFactorial optimization
dc.subjectInsulin delivery
dc.subjectIonotropic gelation
dc.subjectNanoparticles
dc.subjectPolyelectrolyte complexation
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleChitosan/albumin coating factorial optimization of alginate/dextran sulfate cores for oral delivery of insulin
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
relation.isAuthorOfPublicationba32f760-d55c-40a7-9452-62dfd47cbeaf
relation.isAuthorOfPublication.latestForDiscoveryba32f760-d55c-40a7-9452-62dfd47cbeaf
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