Publication: Blind deconvolution of second harmonic microscopy images of the living human eye
Authors
Mugnier, Laurent M. ; Martínez Ojeda, Rosa María ; Artal Soriano, Pablo ; Bueno García, Juan Manuel
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Publisher
Optica Publishing Group
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DOI
https://doi.org/10.1364/BOE.486989
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info:eu-repo/semantics/article
Description
Abstract
Second harmonic generation (SHG) imaging microscopy of thick biological tissues is affected by the presence of aberrations and scattering within the sample. Moreover, additional problems, such as uncontrolled movements, appear when imaging in-vivo. Deconvolution methods can be used to overcome these limitations under some conditions. In particular, we present here a technique based on a marginal blind deconvolution approach for improving SHG images obtained in vivo in the human eye (cornea and sclera). Different image quality metrics are used to quantify the attained improvement. Collagen fibers in both cornea and sclera are better visualized and their spatial distributions accurately assessed. This might be a useful tool to better discriminate between healthy and pathological tissues, especially those where changes in collagen distribution occur.
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Citation
Biomedical Optics Express, 2023, Vol. 14, Issue 5, pp. 2117-2128
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Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by/4.0/