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Changes in alpha-amylase activity, concentration and isoforms in pigs after an experimental acute stress model: an exploratory study

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dc.contributor.authorContreras Aguilar, María Dolores
dc.contributor.authorMartínez Subiela, Silvia
dc.contributor.authorMartínez Miró, Silvia
dc.contributor.authorCerón Madrigal, José Joaquín
dc.contributor.authorTecles Vicente, Fernando
dc.contributor.departmentMedicina y Cirugía Animal
dc.contributor.departmentDepartment of Food and Animal Science, School of Veterinary Medicine, Universitat Autònoma de Barcelona
dc.date.accessioned2024-01-30T10:01:26Z
dc.date.available2024-01-30T10:01:26Z
dc.date.issued2018-08-29
dc.description©2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published version of a Published Work that appeared in final form in BMC Veterinary Research. To access the final edited and published work see https://doi.org/10.1186/s12917-018-1581-2
dc.description.abstractBackground: Salivary alpha-amylase (sAA) is considered a non-invasive biomarker of acute stress that can be evaluated by changes in activity and concentration, and also by changes in its isoforms, although this last way of evaluation has never been used in veterinary medicine. This research evaluated the changes of sAA by three different ways in which sAA can be evaluated in an experimental acute stress model in six pigs based in a technique of temporarily restraining. These ways of evaluation were 1) activity by a spectrophotometric assay, 2) concentration by a fluorometric assay, and 3) isoforms of the enzyme by a Western blot. Results: Although salivary cortisol significantly increased due to the stimulus of stress and all the pigs manifested signs of stress by high-pitched vocalization, sAA activity showed an increase of different degree in the six pigs after the stress stimulus, while sAA concentration showed decreases in four of the six pigs. sAA activity did not correlate with sAA concentration or salivary cortisol, and a low correlation was observed between sAA concentration and salivary cortisol (r= 0.48, p = 0.003). The inter-individual variability was higher in sAA activity than in sAA concentration and salivary cortisol. Finally, three possible isoforms of sAA at 154-160 kDa, 65-66 kDa and 59-60 kDa were observed that showed different dynamics after the stress induction. Conclusions: Although this pilot study's results should be taken with caution due to the low sample size, it reveals a different behavior between sAA activity and concentration in pig after an acute stressful stimulus leading to evident external signs of stress by high-pitched vocalization, and opens a new field for the evaluation of possible selected isoforms of sAA as potential biomarkers of stress.es
dc.formatapplication/pdfes
dc.format.extent8es
dc.identifier.doihttps://doi.org/10.1186/s12917-018-1581-2
dc.identifier.eisbnBMC Veterinary Research, 14, 256es
dc.identifier.issn1746-6148
dc.identifier.urihttp://hdl.handle.net/10201/138118
dc.languageenges
dc.relationÁmbito Nacional, Fundación Séneca, Convocatoria de Ayudas a las Unidades y Grupos de Excelencia Científica de la Región de Murcia (Programa Séneca 2014), 19894/GERM/15es
dc.relation.publisherversionhttps://bmcvetres.biomedcentral.com/articles/10.1186/s12917-018-1581-2es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBiomarkerses
dc.subjectIsoformses
dc.subjectSalivary alpha-amylasees
dc.subjectStresses
dc.subjectSwinees
dc.subject.otherCDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticoses
dc.titleChanges in alpha-amylase activity, concentration and isoforms in pigs after an experimental acute stress model: an exploratory studyes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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