Effect of cumulus cell removal and sperm pre‐incubation with progesterone on in vitro fertilization of equine gametes in the presence of oviductal fluid or cells

Moros-Nicolás, C; Douet, C; Reigner, F; Goudet, G

Publication:
Effect of cumulus cell removal and sperm pre‐incubation with progesterone on in vitro fertilization of equine gametes in the presence of oviductal fluid or cells

dc.contributor.author	Moros-Nicolás, C
dc.contributor.author	Douet, C
dc.contributor.author	Reigner, F
dc.contributor.author	Goudet, G
dc.contributor.department	Biología Celular e Histología
dc.date.accessioned	2024-01-22T13:19:36Z
dc.date.available	2024-01-22T13:19:36Z
dc.date.issued	2019-05-30
dc.description	©2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Reproduction in Domestic Animals. To access the final edited and published work see https://doi.org/10.1111/rda.13479	es
dc.description.abstract	In spite of many attempts to establish an in vitro fertilization (IVF) technique in the equine, no efficient conventional IVF technique is available. The presence of oviductal fluid or oviductal cells during IVF help to improve embryo production in vitro but is not sufficient to reach high fertilization rates. Thus, our aim was to perform equine IVF either after sperm preincubation with oviductal fluid or in the presence of oviductal cells, and to evaluate the effect of cumulus removal from the oocyte or sperm preincubation with progesterone. In experiment 1 and 2, IVF was performed in the presence of porcine oviduct epithelial cells. The removal of cumulus cells from equine oocytes after in vitro maturation tended to increase the percentage of fertilization when fresh sperm was used (1/33 vs 4/31, p > 0.05) but had no effect when frozen sperm was used (1/32 vs 1/32). Equine sperm preincubation with progesterone did not significantly influence the fertilization rate when fresh or frozen sperm was used (2/14 vs 2/18 for fresh, 1/29 vs 1/25 for frozen). In experiment 3 and 4, IVF was performed after preincubation of sperm with porcine oviductal fluid. The removal of cumulus cells tented to increase the percentage of fertilization when fresh sperm was used (1/24 vs 3/26, p>0.05). Sperm preincubation with progesterone did not significantly influence the fertilization rate when fresh or frozen sperm was used (2/39 vs 2/36 for fresh, 2/37 vs 1/46 for frozen), but two 3-4 cells stage zygotes were obtained with fresh sperm preincubated with progesterone. This is an encouraging result for the setting up of an efficient IVF procedure in equine.	es
dc.format	application/pdf	es
dc.format.extent	16	es
dc.identifier.citation	Reproduction in Domestic Animals
dc.identifier.doi	https://doi.org/10.1111/rda.13479
dc.identifier.issn	0936-6768
dc.identifier.issn	1439-0531
dc.identifier.uri	http://hdl.handle.net/10201/137539
dc.language	eng	es
dc.relation	‘Institut Français du Cheval et de l'Equitation’ (IFCE)	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Cumulus	es
dc.subject	IVF	es
dc.subject	Oviduct	es
dc.subject	Oocyte	es
dc.subject	Progesterone	es
dc.subject	Spermatozoa	es
dc.title	Effect of cumulus cell removal and sperm pre‐incubation with progesterone on in vitro fertilization of equine gametes in the presence of oviductal fluid or cells	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es