Publication: Composition of marsupial zona pellucida: a molecular and phylogenetic approach
Authors
Moros-Nicolás, C. ; Chevret, P. ; Izquierdo-Rico, M.J. ; Holt, W.V. ; Esteban-Dían, D. ; López-Béjar, M. ; Martínez-Nevado, E. ; Nilsson, M.A. ; Ballesta, J. ; Avilés, M.
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DOI
https://doi.org/10.1071/RD16519
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info:eu-repo/semantics/article
Description
©2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Accepted version of a Published Work that appeared in final form in Reproduction, Fertility and Development. To access the final edited and published work see https://doi.org/10.1071/RD16519
Abstract
The zona pellucida (ZP) is an extracellular matrix that surrounds mammalian oocytes. In eutherians it is
formed from three or four proteins (ZP1, ZP2, ZP3, ZP4). In the few marsupials that have been studied, however, only three
of these have been characterised (ZP2, ZP3, ZP4). Nevertheless, the composition in marsupials may be more complex,
since a duplication of the ZP3 gene was recently described in one species. The aim of this work was to elucidate the ZP
composition in marsupials and relate it to the evolution of the ZP gene family. For that, an in silico and molecular analysis
was undertaken, focusing on two South American species (gray short-tailed opossum and common opossum) and five
Australian species (brushtail possum, koala, Bennett’s wallaby, Tammar wallaby and Tasmanian devil). This analysis
identified the presence of ZP1 mRNA and mRNA from two or three paralogues of ZP3 in marsupials. Furthermore,
evidence for ZP1 and ZP4 pseudogenes in the South American subfamily Didelphinae and for ZP3 pseudogenes in two
marsupials is provided. In conclusion, two different composition models are proposed for marsupials: a model with four
proteins (ZP1, ZP2 and ZP3 (two copies)) for the South American species and a model with six proteins (ZP1, ZP2, ZP3
(three copies) and ZP4) for the Australasian species
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Reproduction, Fertility and Development
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