Publication: Laser capture microdissection: Big data from small samples
Authors
Datta, Soma ; Malhotra, Lavina ; Dickerson, Ryan ; Chaffee, Scott ; Sen, Chandan K. ; Roy, Sashwati
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Publisher
Universidad de Murcia. Departamento de BiologĂa Celular e HistologĂa
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DOI
10.14670/HH-11-622
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info:eu-repo/semantics/article
Description
Abstract
Any tissue is made up of a heterogeneous
mix of spatially distributed cell types. In response to any
(patho) physiological cue, responses of each cell type in
any given tissue may be unique and cannot be
homogenized across cell-types and spatial co-ordinates.
For example, in response to myocardial infarction, on
one hand myocytes and fibroblasts of the heart tissue
respond differently. On the other hand, myocytes in the
infarct core respond differently compared to those in the
peri-infarct zone. Therefore, isolation of pure targeted
cells is an important and essential step for the molecular
analysis of cells involved in the progression of disease.
Laser capture microdissection (LCM) is powerful to
obtain a pure targeted cell subgroup, or even a single
cell, quickly and precisely under the microscope,
successfully tackling the problem of tissue heterogeneity
in molecular analysis. This review presents an overview
of LCM technology, the principles, advantages and
limitations and its down-stream applications in the fields
of proteomics, genomics and transcriptomics. With
powerful technologies and appropriate applications, this
technique provides unprecedented insights into cell
biology from cells grown in their natural tissue habitat as
opposed to those cultured in artificial petri dish
conditions.
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Citation
Histology and Histopathology, vol. 30, nÂş 11, (2015)
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