Publication: Acetylcholinesterase-positive afferent axons in mucosa of urinary bladder of adult cats: retrograde tracing and degeneration studies
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Date
1995
Authors
wakabayashi, Y. ; Kojima, Y. ; Makiura, Y. ; Tomoyoshi, T. ; Maeda, T.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Acetylcholinesterase (AchE)-positive
afferent axons in the mucosa of the cat urinary bladder
were examined in the present experiments. Smallsized
dorsal root ganglion cells containing AchE
enzyme activity were labelled by injection of retrograde
tracer (wheat germ agglutinin conjugated to
enzymatically inactive horseradish peroxidase gold
complex) into the bladder mucosa of adult cats. Results
show that 48.9% (901184) of the labelled ganglion
cells possessed AchE enzyme activity. Following
unilateral dorsal root ganglionectomy (L2-5, S 1-3), a total
of 6619 unmyelinated axon terminals were examined
in the bladder mucosa, including 691 degenerating
axon terminals. Percentages (8.6- 16.1 %) of degenerating
axon terminals in the ganglionectomized animals (1,
2, 3, 10 and 21 days post-operated) were significantly
higher than those of controls (3.1%) and the 60-day
post-operated animals (3.2%). Approximately onehalf
(47.9%) of the degenerating axon terminals
observed in the 1-21 day post-operated animals were
AchE-positive. Further examination also disclosed that
the population of the intact (not affected by ganglionectomy)
AchE-positive axon terminals at 60 days
(59.3%) was significantly greater than that of controls
(45.6%). The AchE-positive terminals containing few synaptic vesicles were significantly increased
in number in the 60 day post-operated cats. In
conclusion the present study demonstrates that one half
of afferent axons in the mucosa were AchE-positive. The
increase in AchE-positive afferent axon terminals
containing few synaptic vesicles may be derived from
contralateral dorsal root ganglia resulting from sprouting
following dorsal root ganglionectomy.
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