A new highly sensitive immunoassay for the detection of adiponectin in serum and saliva of dogs and its application in obesity and canine leishmaniosis

Muñoz-Prieto, Alberto; Martínez-Subiela, Silvia; Cerón, José Joaquín; Tvarijonaviciute, Asta

Publication:
A new highly sensitive immunoassay for the detection of adiponectin in serum and saliva of dogs and its application in obesity and canine leishmaniosis

dc.contributor.author	Muñoz-Prieto, Alberto
dc.contributor.author	Martínez-Subiela, Silvia
dc.contributor.author	Cerón, José Joaquín
dc.contributor.author	Tvarijonaviciute, Asta
dc.contributor.department	Medicina y Cirugía Animal
dc.date.accessioned	2024-02-09T23:46:34Z
dc.date.available	2024-02-09T23:46:34Z
dc.date.issued	2019-07-31
dc.description.abstract	Adiponectin is an adipokine that exerts insulin-sensitizing and antiinflammatory properties. The aim of this study was to develop and validate a new heterologous ultrasensitive assay based on amplified luminescent technology for adiponectin determination in serum and saliva of dogs. A complete analytical validation of the assay was made in these fluids, and also this assay was applied to quantify adiponectin in serum and saliva of obese and lean dogs and dogs with leishmaniosis. These conditions were selected because in obesity there is a controversy about how adiponectin concentrations change in dogs, and in case of canine leishmaniosis, although it is described a decrease in serum adiponectin, there are not studies about how adiponectin changes after treatment. A total of 11 dogs were used in the validation and 26 dogs with different body condition and 8 with canine leishmaniosis were used for the clinical evaluation of the new assay for adiponectin quantification in serum and saliva of dogs. The analytical evaluation showed that the developed method could measure adiponectin in serum and saliva of dogs with high repeatability and sensitivity, adding a limit of quantification lower than commercially available ELISAs. In addition, significantly higher adiponectin concentrations were found in lean dogs and a correlation between serum and saliva was observed (P < .01). Moreover, dogs with leishmania presented reduced levels of adiponectin in serum. In conclusion, a new assay has been developed for adiponectin measurements which is more sensitive and faster than the traditional ELISA assays requiring less sample volume.	es
dc.embargo.terms	Si
dc.format	application/pdf	es
dc.format.extent	8	es
dc.identifier.doi	https://doi.org/10.1016/j.rvsc.2019.07.019
dc.identifier.eisbn	Research in Veterinary Science	es
dc.identifier.issn	0034-5288
dc.identifier.uri	http://hdl.handle.net/10201/139162
dc.language	eng	es
dc.relation	Sin financiación externa a la Universidad	es
dc.rights	info:eu-repo/semantics/embargoedAccess	es
dc.title	A new highly sensitive immunoassay for the detection of adiponectin in serum and saliva of dogs and its application in obesity and canine leishmaniosis	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es