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Activation of the cell integrity pathway is channelled through diverse signalling elements in fission yeast

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dc.contributor.authorBarba, Gregorio
dc.contributor.authorSoto, Teresa
dc.contributor.authorMadrid, Marisa
dc.contributor.authorNúñez, Andrés
dc.contributor.authorVicente, Jero
dc.contributor.authorGacto, Mariano
dc.contributor.authorCansado Vizoso, José
dc.contributor.departmentGenética y Microbiología
dc.date.accessioned2024-01-31T09:36:08Z
dc.date.available2024-01-31T09:36:08Z
dc.date.issued2008-01-04
dc.description© 2008 Elsevier Inc. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Cellular Signalling. To access the final edited and published work see https://doi.org/10.1016/j.cellsig.2007.12.017es
dc.description.abstractMAPK Pmk1p is the central element of a cascade involved in the maintenance of cell integrity and other functions in Schizosaccharomyces pombe. Pmk1p becomes activated by multiple stressing situations and also during cell separation. GTPase Rho2p acts upstream of the protein kinase C homolog Pck2p to activate the Pmk1 signalling pathway through direct interaction with MAPKKK Mkh1p. In this work we analyzed the functional significance of both Rho2p and Pck2p in the transduction of various stress signals by the cell integrity pathway. The results indicate that basal Pmk1p activity can be positively regulated by alternative mechanisms which are independent on the control by Rho2p and/or Pck2p. Unexpectedly, Pck1p, another protein kinase C homolog, negatively modulates Pmk1p basal activity by an unknown mechanism. Moreover, different elements appear to regulate the stress-induced activation of Pmk1p depending on the nature of the triggering stimuli. Whereas Pmk1p activation induced by hyper- or hypotonic stresses is channeled through Rho2p–Pck2p, other stressors, like glucose deprivation or cell wall disturbance, are transduced via other pathways in addition to that of Rho2p–Pck2p. On the contrary, Pmk1p activation observed during cell separation or after treatment with hydrogen peroxide does not involve Rho2p–Pck2p. Finally, Pck2p function is critical to maintain a Pmk1p basal activity that allows Pmk1p activation induced by heat stress. These data demonstrate the existence of a complex signalling network modulating Pmk1p activation in response to a variety of stresses in fission yeast.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.citationCellular Signalling Vol. 20(4):748-757. 2008
dc.identifier.doihttps://doi.org/10.1016/j.cellsig.2007.12.017
dc.identifier.issnPrint: 0898-6568
dc.identifier.urihttp://hdl.handle.net/10201/138235
dc.languageenges
dc.publisherELSEVIERes
dc.relationÁmbito del proyecto: Nacional. Agencia financiadora: Ministerio de Educación y Ciencia (MEC).Convocatoria: 2005. Nombre del proyecto: Dianas celulares y mecanismos de control del crecimiento regulados por la MAPK Spm1 en S. pombe. Caracterización de los circuitos moleculares que definen su activación Código o número del acuerdo de subvención: BFU2005-01401/BMC. Ámbito del proyecto: Regional. Agencia financiadora: Fundación Séneca (Región de Murcia) .Convocatoria: 2004. Nombre del proyecto: Estudio sobre la activación por estrés de la ruta de MAP quinasas de integridad celular en Schizosaccharomyces pombe. Código o número del acuerdo de subvención: 00475/ PI/04.es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0898656807003944es
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectMicrobiologyes
dc.subjectFission yeastes
dc.subjectMAP kinasees
dc.subjectCell signallinges
dc.subjectCell integrity pathgwayes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiologíaes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísicaes
dc.titleActivation of the cell integrity pathway is channelled through diverse signalling elements in fission yeastes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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