Publication: Ion mobility spectrometry as an emerging tool for characterization of the volatile profile and identification of microbial growth in pomegranate juice
Authors
Castell Martínez, Ana ; Arroyo Manzanares, Natalia ; Hernández, Juan de Dios ; Guillén, Isidro ; Vizcaíno, Pascuali ; López García, Ignacio Francisco ; Hernández Córdoba, Manuel ; Viñas López-Pelegrin, Pilar
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.microc.2021.107099
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info:eu-repo/semantics/article
Description
Abstract
Headspace - gas chromatography (HS-GC) coupled to ion mobility spectrometry (IMS) is proposed as an alternative to plate counting to detect and quantify the microbial contamination in pomegranate juice. Thus, contaminated samples by the yeast Saccharomyces cerevisiae were monitored during 6 non-consecutive days over two weeks using two types of preservatives (a sorbate/benzoate mixture and a natural preservative from vegetable extracts). IMS is an emerging technique with high potential for volatile organic compounds (VOCs) monitoring because its high sensitivity and separation power of ions. The fingerprint of the samples allowed to establish difference in the volatile composition of contaminated and non-contaminated samples. Ethyl acetate, ethyl butyrate and limonene were characterized obtaining limits of detection and quantification below 0.029 and 0.097 μg/g, respectively. Furthermore, chemometric models were performed to detect contaminated pomegranate juice and to assess the concentration of yeast, obtaining a validation success of 100 and 90.91%, respectively.
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Citation
Microchemical Journal, 2022, Vol. 174 : 107099
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