Publication: Chitosan-based nanocapsules: physical characterization, stability in biological media and capsaicin encapsulation
Authors
Goycoolea Valencia, Francisco Martín ; Angela Valle-Gallego ; Rossana Stefani ; Bianca Menchicchi ; Laurent David ; Cyrille Rochas ; Manuel J. Santander-Ortega ; María J. Alonso
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Facultades de la UMU::Facultad de Biología
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Publisher
Springer-Verlag
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DOI
https://doi.org/10.1007/s00396-012-2669-z
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info:eu-repo/semantics/article
Description
Abstract
This study addresses the effect of the degree of Nacetylation (DA ~1.4–56 %) of chitosan (CS) of low and medium molecular weight (Mw ~9.5–13.2 and ~122–266 kDa, respectively) on the biophysical properties, colloidal stability and encapsulation efficiency of capsaicin (a lipophilic drug currently used in pain therapy) of CS-based nanocapsules (NCs). The average diameter of the NCs varied within the range of ~150–200 nm, but it was slightly higher for NCs comprising high Mw CS than for those with low Mw CS. The zeta potential (ζ) was highly positive and clearly dependent on the Mw of CS, exhibiting a monotonic decreasing trend concomitant with the increase in DA. Both results suggest that Mw and DA of CS have an effect on the disposition of the polysaccharide onto the phospholipidic surface. Synchrotron SAXS studies revealed a monotonic increase in Bragg interplanar distances (from ~55 up to ~67 Å) as a function of the DA, a finding that agrees with previous results that are consistent with the capacity of hydrophobic domains to disturb the crystalline state ofgelled phospholipidic membranes. Colloidal stability studies carried out in cell-culture biological media revealed the determinant role of hydration forces, a short-range repulsive interaction, on the stability of the NCs. Finally, Mw and DA of CS both influenced the encapsulation efficiency of capsaicin, thus showing the effect of the harnessed NCs shell on its capacity to encapsulate lipophilic drugs.
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Citation
Goycoolea F.M., Valle-Gallego A., Stefani R., Menchicchi B., David L., Rochas C., Santander-Ortega M.J., Alonso M.J. (2012). Chitosan-based nanocapsules: physical characterization stability in biological media and capsaicin encapsulation. Colloid Polym Sci, 290, 1423-1434
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