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A smart strategy to Improve t-Resveratrol production in grapevine cells treated with Cyclodextrin polymers coated with magnetic nanoparticles

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dc.contributor.authorAlmagro Romero, Lorena
dc.contributor.authorGea Abellán, Alicia de
dc.contributor.authorRodríguez López, Marïa Isabel
dc.contributor.authorNúñez Delicado, Estrella
dc.contributor.authorGabaldón, José Antonio
dc.contributor.authorPedreño, María Ángeles
dc.contributor.departmentBiología Vegetal
dc.date.accessioned2025-01-29T16:15:00Z
dc.date.available2025-01-29T16:15:00Z
dc.date.issued2020-04-24
dc.description© 2020 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published Manuscript version of a Published Work that appeared in final form in Polymers. To access the final edited and published work see https://doi.org/10.3390/polym12040991
dc.description.abstractOne of the most successfully procedures used to increase the production of t-resveratrol in Vitis vinifera suspension-cultured cells is the application of cyclodextrins (CDs) and methyl jasmonate (MJ) as elicitors. In particular, β-CDs are characterized by their chemical structure which makes them special, not only by acting as elicitors, but also because they are compounds capable of trapping high added-value hydrophobic molecules such as t-resveratrol. However, the use of β-CDs as elicitors increases the production costs of this compound, making their industrial exploitation economically unfeasible. Therefore, the development of β-CDs recovery strategies is necessary to provide a viable solution to their industrial use. In this work, carboxymethylated and hydroxypropylated β-CDs have been used to form polymers using epichlorohydrin (EPI) as a cross-linking agent. The polymers were coated to Fe3O4 nanoparticles and were jointly used with MJ to elicit V. vinifera suspension-cultured cells. Once elicitation experiments were finished, a magnet easily allowed the recovery of polymers, and t-resveratrol was extracted from them by using ethyl acetate. The results indicated that the production of t-resveratrol in the presence of free carboxymethyl-β-CDs was much lower than that found in the presence of carboxymethyl-β-cyclodextrins-EPI polymer coated magnetic nanoparticles. In addition, the maximal levels of t-resveratrol were found at 168 h of elicitation in the presence of 15 g/L hydroxypropyl-β-CDs polymer coated magnetic nanoparticles and MJ, and non-t-resveratrol was found in the extracellular medium, indicating that all the t-resveratrol produced by the cells and secreted into the culture medium was trapped by the polymer and extracted from it. This work also showed that polymers can be regenerated and reused during three cycles of continuous elicitation since the induction and adsorption capacity of hydroxypropyl-β-CDs polymer-coated magnetic nanoparticles after these cycles of elicitation remained high, allowing high concentrations of t-resveratrol to be obtained.es
dc.formatapplication/pdfes
dc.format.extent14es
dc.identifier.doihttps://doi.org/10.3390/polym12040991
dc.identifier.eisbnPolymers, 2020, Vol. 12, Issue 4 : 991es
dc.identifier.issnElectronic: 2073-4360
dc.identifier.urihttp://hdl.handle.net/10201/149646
dc.languageenges
dc.publisherMDPIes
dc.relationThis research was funded by Ministerio de Economía y Competitividad (Grant number: BIO2017-82374-R) and Fundación Seneca-Agencia de Ciencia y Tecnología de la Región de Murcia (Grant number: 19876/GERM/15).es
dc.relation.publisherversionhttps://www.mdpi.com/2073-4360/12/4/991es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCarboxymethyl β cyclodextrin polymer coated magnetic nanoparticleses
dc.subjectHydroxypropyl β cyclodextrin polymer coated magnetic nanoparticleses
dc.subjectT resveratroles
dc.subjectMethyl jasmonatees
dc.subjectVitis vinifera suspension cultured cellses
dc.titleA smart strategy to Improve t-Resveratrol production in grapevine cells treated with Cyclodextrin polymers coated with magnetic nanoparticleses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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