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Antibacterial potential of commercial and wild lactic acid bacteria strains isolated from ovine and caprine raw milk against Mycoplasma agalactiae

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dc.contributor.authorToquet, Marion
dc.contributor.authorBataller, Esther
dc.contributor.authorGomis Almendro, Jesús
dc.contributor.authorSánchez López, Antonio
dc.contributor.authorToledo-Perona, Raquel
dc.contributor.authorde la Fe Rodríguez, Christian
dc.contributor.authorCorrales Romero, Juan Carlos
dc.contributor.authorGómez-Martin, Ángel
dc.contributor.departmentSanidad Animal
dc.date.accessioned2023-11-22T07:18:44Z
dc.date.available2023-11-22T07:18:44Z
dc.date.issued2023-06-22
dc.description© 2023 Toquet, Bataller, Gomis, Sánchez, Toledo-Perona, De la Fe, Corrales and Gómez-Martín. This is an open-access article distributed under the terms of the Creative Commons CC-BY license, http://creativecommons.org/licenses/by /4.0/ This document is the Published version of a Published Work that appeared in final form in Frontiers in Veterinary Science. To access the final edited and published work see https://doi.org/10.3389/fvets.2023.1197701es
dc.description.abstractIntroduction: The complexity of fighting contagious agalactia (CA) has raised the necessity of alternative antimicrobial therapies, such as probiotics. Lactic acid bacteria (LAB) are present in the mammary gland of small ruminants and their antimicrobial effect have been previously described against species like Mycoplasma bovis but never against Mycoplasma agalactiae (Ma). This in vitro study aims to evaluate the antimicrobial activity against Ma of ovine and caprine LAB strains and a human commercial probiotic (L2) of Lactobacillus spp. Methods: A total of 63 possible LAB strains were isolated from nine ovine and caprine farms in Spain, three isolates (33B, 248D, and 120B) from the 63 strains were selected, based on their capacity to grow in a specific medium in vitro, for an in vitro experiment to assess their antimicrobial activity against Ma in Ultra High Temperature (UHT) processed goat milk (GM). A women commercial vaginal probiotic was also included in the study. The inoculum of L2 was prepared at a concentration of 3.24 × 108  CFU/mL and the average concentration of the inoculum of the wild LAB varied from 7.9 × 107 to 8.4 × 108  CFU/mL. Results: The commercial probiotic L2 significantly reduced the concentration of Ma to 0.000 log CFU/mL (p < 0.001), strain 33B reduced it from 7.185 to 1.279 log CFU/mL (p < 0.001), and 120B from 6.825 to 6.466 log CFU/mL (p < 0.05). Strain 248D presented a bacteriostatic effect in GM. Moreover, the three wild strains and the commercial probiotic produced a significative reduction of the pH (p < 0.001). Discussion: This is the first in vivo report of the antimicrobial potential of LAB strains against Ma and its interaction. Our results support possible future alternative strategies to antibiotic therapy, previously not contemplated, to fight CA in small ruminants. Further studies are necessary to elucidate the action mechanisms through which these LAB are able to inhibit Ma and to assess the safety of using these strains in possible in vivo studies.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.doihttps://doi.org/10.3389/fvets.2023.1197701
dc.identifier.eisbnFrontiers in Veterinary Science, Volume 10 - 2023 10:1197701.es
dc.identifier.issn2297-1769
dc.identifier.urihttp://hdl.handle.net/10201/135929
dc.languageenges
dc.publisherFrontiers Mediaes
dc.relationThis work was supported by Generalitat Valenciana (Spain; GVA/2020/026) and the Spanish Ministry of Science and Innovation (PID2020-119462RA-I00/AEI/10.13039/501100011033).es
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fvets.2023.1197701/fulles
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectLactobacilluses
dc.subjectEnterococcuses
dc.subjectMastitises
dc.subjectContagious agalactiaes
dc.subjectAntimicrobial activityes
dc.subjectMycoplasma agalactiaees
dc.subjectProbiotices
dc.subjectRaw milkes
dc.titleAntibacterial potential of commercial and wild lactic acid bacteria strains isolated from ovine and caprine raw milk against Mycoplasma agalactiaees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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