Characterization of macrophages from the bony fish gilthead seabream using an antibody against the macrophage colony-stimulating factor receptor

Mulero Méndez, Iván; Sepulcre Cortés, María Pilar; Roca Soler, Francisco José; Meseguer Peñalver, J.; Garcia-Ayala, Alfonsa; Mulero Méndez, Victoriano Francisco

Publication:
Characterization of macrophages from the bony fish gilthead seabream using an antibody against the macrophage colony-stimulating factor receptor

dc.contributor.author	Mulero Méndez, Iván
dc.contributor.author	Sepulcre Cortés, María Pilar
dc.contributor.author	Roca Soler, Francisco José
dc.contributor.author	Meseguer Peñalver, J.
dc.contributor.author	Garcia-Ayala, Alfonsa
dc.contributor.author	Mulero Méndez, Victoriano Francisco
dc.contributor.department	Bioquímica y Biología Molecular B e Inmunología
dc.date.accessioned	2025-01-29T16:54:36Z
dc.date.available	2025-01-29T16:54:36Z
dc.date.issued	2008-04-07
dc.description	© 2008 Elsevier Ltd. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Developmental and Comparative Immunology. To access the final edited and published work see https://doi.org/10.1016/j.dci.2008.03.005
dc.description.abstract	Two major professional phagocyte populations have been described in fish, namely granulocytes and monocytes/macrophages. Although the distribution and localization of macrophages have been documented in several teleost species using mainly light and/or electron microscopy, the lack of appropriate markers for these cells has hampered our in-depth knowledge of their biology. We report here the generation of a monospecific rabbit polyclonal antibody against the gilthead seabream macrophage colony-stimulating factor receptor (Mcsfr), which is an excellent marker of macrophages in mammals and the zebrafish. The anti-Mcsfr has been found to be very useful in immunohistochemistry (IHC) to specifically immunostain the purified macrophages (adherent cells) obtained from the head-kidney as well as different cell populations in paraffin-embedded organs, including the head-kidney, spleen, thymus, gills and liver. Unexpectedly, however, no Mcsfr immunoreactive (Mcsfr+) cells were observed in the brain and intestine of the gilthead seabream. We also show that the distribution of Mcsfr+ cells in the head-kidney and the spleen is unaltered following infection with the fish pathogenic bacterium Vibrio anguillarum and that the Il1b-producing cells in these two organs after infection are exclusively acidophilic granulocytes. Finally, as the epitope recognized by the anti-Mcsfr is well conserved, we illustrate the potential usefulness of this antibody in other teleost species, such as the European seabass.	es
dc.embargo.terms	Si
dc.format	application/pdf	es
dc.format.extent	9	es
dc.identifier.doi	https://doi.org/10.1016/j.dci.2008.03.005
dc.identifier.eisbn	Developmental and Comparative Immunology (2008) 32, 1151–1159	es
dc.identifier.issn	Print: 0145-305X
dc.identifier.issn	Electronic: 1879-0089
dc.identifier.uri	http://hdl.handle.net/10201/149653
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	Spanish Ministry of Education and Science (Grant BIO2005-05078 to V.M.), Consejería de Educación y Cultura de la Comunidad Autónoma de la Región de Murcia (Grant 08BIO2005-01-6471 to V.M.), and Fundación Séneca-Murcia (fellowship to I.M.)	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S0145305X08000621?via%3Dihub
dc.rights	info:eu-repo/semantics/embargoedAccess	es
dc.subject	Innate immunity	es
dc.subject	Infection	es
dc.subject	Vibrio anguilarum	es
dc.subject	European seabass	es
dc.subject	Acidophilic
dc.subject	Granulocytes
dc.subject	Interleukin-1
dc.subject	Teleosts
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología	es
dc.title	Characterization of macrophages from the bony fish gilthead seabream using an antibody against the macrophage colony-stimulating factor receptor	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es