Collado-González,  Mar

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Collado-González, Mar

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Collado-González, Mar

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Universidad de Murcia. Departamento de Biología Celular e Histología

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Open Access
Description and comparative study of physico-chemical parameters of the teleost fish skin mucus.
(SAGE Publications, 2015-07-01) Guardiola Abellán, Francisco Antonio; Cuartero, María; Collado-González, Mar; Arizcún, Marta; Díaz Baños, F. Guillermo; Meseguer, José; Esteban Abad, María de los Ángeles; Biología Celular e Histología
The study of mucosal surfaces, and in particular the fish skin and its secreted mucus, has been of great interest recently among immunologists. Measurement of the viscosity and other physico-chemical parameters (protein concentration, pH, conductivity, redox potential, osmolality and density) of the skin mucus can help to understand its biological functions. We have used five marine species of teleost: gilthead seabream (Sparus aurata L.), European sea bass (Dicentrarchus labrax L.), shi drum (Umbrina cirrosa L.), common dentex (Dentex dentex L.) and dusky grouper (Epinephelus marginatus L.), all of them with commercial interest in the aquaculture of the Mediterranean area. Mucus showed a direct shear- and temperature-dependent viscosity, with a non-Newtonian behavior, which differed however between two groups: one with higher viscosity (D. labrax, U. cirrosa, D. dentex) and the other with lower viscosity (S. aurata, E. marginatus). In addition, there was a clear interrelation between density and osmolality, as well as between density and temperature. Taking into account that high values of viscosity should improve the barrier effect against pathogens but low values of viscosity are needed for good locomotion characteristics, our results may help elucidate the relationship between physico-chemical and biological parameters of skin mucus, and disease susceptibility.
Open Access
Silk fibroin and graphene oxide composites promote human periodontal ligament stem cell spontaneous differentiation into osteo/cementoblast-like cells
(Mary Ann Liebert, Inc, 2016-11-15) Sánchez Vera, María del Mar; Aznar-Cervantes, Salvador; Jover, Eva; García Bernal, David; Oñate Sánchez, Ricardo Elías; Hernández Romero, Diana; Moraleda Jiménez, José María; Collado-González, Mar; Rodríguez Lozano, Francisco Javier; Cenís, José L.; Dermatología, Estomatología, Radiología y Medicina Física
Graphene represents one of the most interesting additions to the tissue engineering toolbox. Novel graphenebased composites are required to improve the beneficial graphene properties in terms of tridimensional polymeric structure, conferring a higher mechanical strength and favoring the differentiation of human mesenchymal stem cells. Here, we have demonstrated in a wide range of composite combinations, the successful use of graphene and silk-fibroin constructs for future bioengineering applications in the field of clinical regenerative dentistry using human periodontal ligament stem cells. Our results provide exciting new data for the development of suitable scaffolds that allow good cell engrafting, preservation of cell viability and proliferation, promotion of spontaneous osteoblastic differentiation, and importantly, stimulation of a higher cementum physiological synthesis than using other different available biomaterials.
Open Access
Citotoxicity of Guttaflow bioseal, Guttaflow2, MTA Fillapex, and AH Plus on human periodontal ligament stem cells.
(Elsevier Science, 2017-03-23) Collado-González, Mar; Tomás Catalá, Christopher Joseph; Oñate Sánchez, Ricardo Elías; Moraleda Jiménez, José María; Rodríguez Lozano, Francisco Javier; Dermatología, Estomatología, Radiología y Medicina Física
Introduction: The aim of the present study was to evaluate the in vitro cytotoxicity of endodontic sealers (GuttaFlow Bioseal, GuttaFlow2, and MTA Fillapex) on human periodontal ligament stem cells (hPDLSCs). As a reference, AH Plus was compared with the more recent endodontic sealers regarding cell viability and cell attachment. Methods: Biological testing was carried out in vitro on hPDLSCs. Cell viability assay was performed by using eluates from each endodontic sealer. To assess cell orphology and attachment to the different sealers, the hPDLSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. Chemical composition of the sealers was determined by energy-dispersive x-ray, and eluates were analyzed by inductively coupled plasma mass spectrometry. Statistical differences were assessed by analysis of variance and Tukey test (P < .05). Results: Cell viability was evident after 24 hours in the presence of GuttaFlow Bioseal and GuttaFlow 2 but not in the case of AH Plus or MTA Fillapex. At 168 hours, GuttaFlow Bioseal and GuttaFlow 2 exhibited high and moderate cell viability, respectively, whereas AH Plus and MTA Fillapex revealed low rates of cell cell viability (P < .001). Finally, scanning electron microscopy studies revealed a high degree of proliferation, cell spreading, and attachment, especially when using GuttaFlow Bioseal disks. Conclusions: GuttaFlow Bioseal and GuttaFlow2 showed lower cytotoxicity than MTA Fillapex and AH plus. Further in vitro and in vivo investigations are required to confirm the suitability of GuttaFlow Bioseal for clinical application.
Open Access
Complex polysaccharide-based nanocomposites for 2 oral insulin delivery
(MDPI (Basel, Switzerland), 2020-01-15) Collado-González, Mar; Freitas, Alessandra R.; Santos, Ana Claudia; Ferreira, Nuno F.; Ferreri, Maria Cristina; Sequeira, Joana A.D.; Díaz Baños, F. Guillermo; Víllora Cano, Gloria; Veiga, Francisco; Ribeiro, Antonio; Carissimi, Guzmán; Química Física
Polyelectrolyte nanocomposites rarely reach a stable state and aggregation often occurs. Here, we report the synthesis of nanocomposites for the oral delivery of insulin composed of alginate, dextran sulfate, poly-(ethyleneglycol) 4000, Poloxamer 188®, chitosan, and BSA. Chitosan seems to be essential for the final size of the nanocomposites. When the chitosan concentration used in the synthesis is 10-4 gcm-3, nanocomposites, microcomposites and milimetric composites are obtained. Reducing the chitosan concentration to 10-5 or 10-7 gcm-3 yields nanocomposites of 400-600 nm or 800-900 nm hydrodynamic diameter, respectively, measured by DLS. According to our results, the molecular weight of chitosan has little influence on the size of the composites. The enhanced stability of the newest nanocomposites synthesized was assessed with LUMiSizer®. The rounded shapes of the nanocomposites were confirmed by scanning electron microscopy. Analysis by HPLC revealed that, after loaded with insulin, about 80% of the drug was released from our nanocomposites.
Open Access
Biocompatibility of three new calcium silicate-based endodontic sealers on human periodontal ligament stem cells
(Willey, 2016-09-26) Collado-González, Mar; García Bernal, David; Oñate Sánchez, Ricardo Elías; Ortolani-Seltenerich, P.S.; Lozano, Adrián; Forner, Leopoldo; Llena, Carmen; Rodríguez Lozano, Francisco Javier; Dermatología, Estomatología, Radiología y Medicina Física
Aim To evaluate the biocompatibility of three calcium silicate-based endodontic sealers, Bioroot BC Sealer (Septodont, Saint-Maur-des-Fosses, France), Endoseal MTA (EndoSeal, Maruchi, Seoul, Korea) and Nano-ceramic Sealer (B&L Biotech, Fairfax, VA, USA) (NCS), on human periodontal ligament stem cells (hPDLSCs). Methodology human periodontal ligament stem cells were cultured in the presence of various endodontic sealer eluates for 24 h. Cell viability was determined using the MTT assay. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. Also, an in vitro scratch wound-healing model was used to determine their effects in cell migration. Finally, to assess cell morphology and attachment to the different sealers, hPDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy (SEM). One-way analysis of variance (ANOVA) followed by a Bonferroni post-test was performed (P < 0.05). Results At 24 h, cell spreading was evident in the presence of Bioroot BC Sealer (BR) and Nano-ceramic Sealer (NCS), but not Endoseal MTA (ES). At 72 h, BR and NCS exhibited high and moderate cell proliferation, respectively, whereas ES revealed low rates of cell proliferation (P < 0.05). Similar results were obtained in a cell death assay. In addition, hPDLSCs maintained their mesenchymal phenotype in all conditions although their capacity to migrate was higher in the presence of BR. Finally, SEM studies revealed a good degree of proliferation, cell spreading and attachment, especially when using BR and NCS discs. Conclusions BR and NCS were associated with better cytocompatibility than ES. Further in vitro and in vivo investigations are required to confirm the suitability of these calcium silicate-based endodontic sealers for clinical application.
Open Access
Resveratrol lacks antifungal activity against Candida albicans.
(Springer, 2012-03-30 ) Collado-González, Mar; Guirao-Abad, José Pedro; Sánchez-Fresneda Pinto, Ruth; Belchí Navarro, Sarai; Argüelles Ordóñez, Juan Carlos; Genética y Microbiología
The putative candicidal activity of resveratrol is currently a matter of controversy. Here, the antifungal activity as well as the antioxidant response of resveratrol against Candida albicans, have been tested in a set of strains with a well-established genetic background At the doses usually employed in antifungal tests (10–40 lg/ml), resveratrol has no effect on the exponential growth of the C. albicans CAI.4 strain, a tenfold increase (400 lg/ml) was required in order to record a certain degree of cell killing, which was negligible in comparison with the strong antifungal effect caused by the addition of amphotericin B (5 lg/ml). An identical pattern was recorded in the prototrophic strains of C. albicans SC5314 and RM-100, whereas the oxidative sensitive trehalose-deficient mutant (tps1/tps1 strain) was totally refractory to the presence of resveratrol. In turn, the serum-induced yeast-to-hypha transition remained unaffected upon addition of different concentrations of resveratrol. Determination of endogenous trehalose and catalase activity, two antioxidant markers in C. albicans; revealed no significant changes in their basal contents induced by resveratrol. Collectively, our results seem to dismiss a main antifungal role as well as the therapeutic application of resveratrol against the infections caused by C. albicans.
Open Access
Nanotechnology-based formulations for resveratrol delivery: Eﬀects on resveratrol in vivo bioavailability and bioactivity
(Elsevier Sci Ltd, 2019-04-15) Collado-González, Mar; Pereira, Irina; Pereira-Silva, Miguel; Ferreira, Laura; Caldas, Mariana; Magalhães, Mariana; Figueiras, Ana; Ribeiro, António J.; Veiga, Francisco; Santos, Ana Claudia; Biología Celular e Histología
Resveratrol (RES), also known as 3,5,4′-trihydroxystilbene, is a polyphenolic phytoalexin that has been widely researched in the past decade due to its recognized numerous biological activities. Despite the potential benefits of RES, its effective use is limited due to its poor solubility, photosensitivity and rapid metabolism, which strongly undermine RES bioavailability and bioactivity. Thereby, recently, nanotechnology appeared as a po- tential strategy to circumvent RES physicochemical and pharmacokinetics constrains. However, only few studies have addressed the crucial in vivo suitability of the developed delivery systems to improve RES efficacy. Facing this scenario, in the present review, it is intended to present and discuss the in vivo resveratrol bioavailability and bioactivity, following its encapsulation or conjugation in nanotechnology-based carriers, contemplating their pharmacokinetics effectiveness.
Open Access
Modification of chicha gum: antibacterial activity, ex vivo mucoadhesion, antioxidant activity and cellular viability
(Elsevier, 2022-12-21) Freitas, Alessandra R.; Ribeiro, António J.; Ribeiro, Alessandra B.; Collado-González, Mar; Silva, Luís R.; Alves, Luís; Melro, Elodie; Antunes, Filipe E.; Veiga, Francisco; Morais, Alan I. S.; Bezerra, Roosvelt D. S.; Soares-Sobrinho, José L.; Osajima, Josy A.; Silva-Filho, Edson C.; Biología Celular e Histología
The aim of the present work was to modify the exuded gum of Sterculia striata tree by an amination reaction. The viscosity and zero potential of the chicha gum varied as a function of pH. The modification was confirmed by Xray diffraction (XRD), infrared spectroscopy (FTIR), size exclusion chromatography (SEC), zeta potential, thermogravimetric analysis (TG), and differential scanning calorimetry (DSC). Furthermore, the chemical modification changed the molar mass and surface charge of the chicha gum. In addition, the gums were used in tests for ex vivo mucoadhesion strength, antibacterial activity against the standard strain of Staphylococcus aureus (ATCC 25923), inhibitory activity of α-glucosidase, antioxidant capacity, and viability of Caco-2 cells. Through these tests, it was found that amination caused an increase in the mucoadhesive and inhibitory activity of chicha gum against the bacterium Staphylococcus aureus. In addition, the gums (pure and modified) showed antioxidant capacity and an inhibitory effect against the α-glucosidase enzyme and did not show cytotoxic potential.
Open Access
Comparison of sulfamethoxazole removal efficiency using polyethersulfone ultrafiltration membrane modified by various methods
(MDPI, 2024-12-20) Hidalgo Montesinos, Asunción María; Murcia Almagro, María Dolores; Gómez Gómez, María; Collado-González, Mar; Montiel Morte, María Claudia; Martínez Morga, Marta; Martínez, Marta; Ingeniería Química ; Biología Celular e Histología
Nowadays, there is a growing interest in membrane modification processes to improve their characteristics and the effectiveness of their treatments and reduce the possible fouling. In this sense, in this work, a modification of an ultrafiltration membrane with three different materials has been carried out: reduced graphene oxide (rGO), chitosan and MgCl2. For both the native and the modified membranes, a study has been carried out to remove the emerging contaminant sulfamethoxazole (SMX). SEM and SEM-EDX analyses have been performed to confirm membrane surface modifications. In the characterisation of the membranes, it is noteworthy that the values of the permeability coefficient, Aw, have been lower in the modified membranes, which is unexpected. Regarding the pollutant removal tests, the influence of pressure and initial concentration on permeate flux and rejections has been studied. Native membrane shows the highest permeate flux values. Comparing the modified membranes, the highest rejection values are obtained with the rGO-modified membrane, which can be explained by its greater hydrophilic character. Finally, a fouling study was carried out, verifying that in almost all cases, fouling occurs after the passage of the pollutant due to the blockage of the membrane pores.
Embargo
Evaluation of changes in ion release and biological properties of NeoMTA-Plus and Endocem-MTA exposed to an acidic environment
Rodríguez Lozano, Francisco Javier; Oñate Sánchez, Ricardo E.; Lopez-García, S.; García-Bernal, D.; Moraleda, J. M.; Lozano, A.; Forner, L.; Murcia, L.; Collado-González, Mar; Dermatología, Estomatología, Radiología y Medicina Física
Aim To analyse in vitro changes in ion release and biological properties of Endocem-MTA (Maruchi, Wonju, Korea) and NeoMTA-Plus (Avalon Biomed Inc, Bradenton, FL, USA) exposed to acidic or neutral environment on human dental periodontal ligament stem cells (hPDLSCs). Methodology Cell viability and wound healing assays were performed using eluates of each material. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. To evaluate cell attachment to the different materials, hPDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy. The chemical composition of the materials was determined by energy-dispersive X-ray (EDX), and ion release was evaluated by inductively coupled plasma-mass spectrometry. Statistical analysis was performed with analysis of variance and a Bonferroni or Tukey post-test (a < 0.05). Results The MTT assay revealed non-cytotoxic effects of NeoMTA-Plus and Endocem-MTA at pH 5.2 and 7.4. However, there were minor differences compared with the control, especially at pH 5.2, where both materials were associated with significantly greater cell viability (P < 0.05). In both environments, the materials stimulated hPDLSCs to migrate. hPDLSCs were attached to the bioactive cements, with multiple prolongations proliferated on the surface of the samples. Moreover, there were no changes to cell phenotype or apoptosis/necrosis rates, indicating that the acidic environment did not induce cell death. Prismatic crystalline structures were seen on the surface of the cements exposed to butyric acid and EDX analysis identified a marked peak of Ca2+ from NeoMTA-Plus and Endocem-MTA in acidic and physiological environments. Conclusions An acidic environment favoured the release of Ca2+ ions from both bioactive cements, and the cytotoxicity of these bioactive cements was low in both environments studied.