Person: Bueno García, Juan Manuel
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Bueno García
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Juan Manuel
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Bueno García, Juan Manuel
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- PublicationOpen AccessAssessment of the corneal collagen organization after chemical burn using second harmonic generation microscopy(Optica Publishing Group, 2021-01-11) Bueno García, Juan Manuel; Ávila, Francisco J.; Lorenzo-Martín, Elvira; Gallego-Muñoz, Patricia; Martínez-García, M. Carmen; FísicaThe organization of the corneal stoma is modified due to different factors, including pathology, surgery or external damage. Here the changes in the organization of the corneal collagen fibers during natural healing after chemical burn are investigated using second harmonic generation (SHG) imaging. Moreover, the structure tensor (ST) was used as an objective tool for morphological analyses at different time points after burn (up to 6 months). Unlike control corneas that showed a regular distribution, the collagen pattern at 1 month of burn presented a non-organized arrangement. SHG signal levels noticeably decreased and individual fibers were hardly visible. Over time, the healing process led to a progressive re-organization of the fibers that could be quantified through the ST. At 6 months, the stroma distribution reached values similar to those of control eyes and a dominant direction of the fibers re-appeared. The present results show that SHG microscopy imaging combined with the ST method is able to objectively monitor the temporal regeneration of the corneal organization after chemical burn. Future implementations of this approach into clinically adapted devices would help to diagnose and quantify corneal changes, not only due to chemical damages, but also as a result of disease or surgical procedures.
- PublicationOpen AccessGalvanic current activates the NLRP3 inflammasome to promote Type I collagen production in tendon(eLife Sciences Publications, 2022-02-24) Peñin Franch, Alejandro; García Vidal, José Antonio; Martínez Cáceres, Carlos Manuel; Escolar Reina, Pilar; Martínez Ojeda, Rosa M. ; Gómez, Ana I.; Bueno García, Juan Manuel; Minaya-Muñoz, Francisco; Valera-Garrido, Fermín; Escolar Reina, Pilar; Bueno García, Juan Manuel; Medina i Mirapeix, Francesc; FisioterapiaThe NLRP3 inflammasome coordinates inflammation in response to different pathogen- and damage-associated molecular patterns, being implicated in different infectious, chronic inflammatory, metabolic and degenerative diseases. In chronic tendinopathic lesions, different non-resolving mechanisms produce a degenerative condition that impairs tissue healing and which therefore complicates their clinical management. Percutaneous needle electrolysis consists of the application of a galvanic current and is an emerging treatment for tendinopathies. In the present study, we found that galvanic current activates the NLRP3 inflammasome and induces an inflammatory response that promotes a collagen-mediated regeneration of the tendon in mice. This study establishes the molecular mechanism of percutaneous electrolysis that can be used to treat chronic lesions and describes the beneficial effects of an induced inflammasome-related response.
- PublicationOpen AccessArrangement of the photoreceptor mosaic in a diabetic rat model imaged with multiphoton microscopy(Frontiers Media, 2020-08-07) Bueno García, Juan Manuel; Cruz-Castillo, Ricardo; Avilés-Trigueros, Marcelino; Bautista-Elivar, Nazario; FísicaDiabetic retinopathy (DR) is defined as a microvascular pathology. However, some data have suggested that the retinal photoreceptors (PRs) might be important in the pathogenesis of this ocular disease. In this study the organization of the PRs in control and diabetic-induced rats was compared using multiphoton microscopy. The PR mosaic was imaged at different locations in non-stained retinas. The density of PRs was directly quantified from cell counting. The spatially resolved density presents a double-slope pattern (from the central retina towards the periphery) in both healthy and pathological samples, although the values for the latter were significantly lower all across the retinas. Moreover, Voronoi analysis was performed to explore changes in PR topography. In control specimens a hexagonally packed structure was dominant. However, despite the non-controlled effects of the disease in retinal structures, this PR regularity was fairly maintained in diabetic retinas.
- PublicationOpen AccessFluorescent bicolour sensor for low-background neutrinoless double β decay experiments(Nature Research, 2020-06-22) Rivilla, Iván; Aparicio, Borja; Bueno García, Juan Manuel; Casanova, David; Tonnelé, Claire; Freixa, Zoraida; Herrero, Pablo; Rogero, Celia; Miranda, José I.; Martínez-Ojeda, Rosa M.; Monrabal, Francesc; Olave, Beñat; Schäfer, Thomas; Artal, Pablo; Nygren, David; Cossío, Fernando P.; Gómez-Cadenas, Juan J.; FísicaObservation of the neutrinoless double β decay is the only practical way to establish that neutrinos are their own antiparticles1. Because of the small masses of neutrinos, the lifetime of neutrinoless double β decay is expected to be at least ten orders of magnitude greater than the typical lifetimes of natural radioactive chains, which can mimic the experimental signature of neutrinoless double β decay2. The most robust identification of neutrinoless double β decay requires the definition of a signature signal—such as the observation of the daughter atom in the decay—that cannot be generated by radioactive backgrounds, as well as excellent energy resolution. In particular, the neutrinoless double β decay of 136Xe could be established by detecting the daughter atom, 136Ba2+, in its doubly ionized state3–8. Here we demonstrate an important step towards a ‘barium-tagging’ experiment, which identifies double β decay through the detection of a single Ba2+ ion. We propose a fluorescent bicolour indicator as the core of a sensor that can detect single Ba2+ ions in a high-pressure xenon gas detector. In a sensor made of a monolayer of such indicators, the Ba2+ dication would be captured by one of the molecules and generate a Ba2+-coordinated species with distinct photophysical properties. The presence of such a single Ba2+-coordinated indicator would be revealed by its response to repeated interrogation with a laser system, enabling the development of a sensor able to detect single Ba2+ ions in high-pressure xenon gas detectors for barium-tagging experiments.
- PublicationOpen AccessQuantitative structural organization of the sclera in chicks after deprivation myopia measured with second harmonic generation microscopy(Frontiers Media, 2024-10-22) Bueno García, Juan Manuel; Martínez-Ojeda, Rosa M.; Fernández, Enrique J.; Feldkaemper, Marita; FísicaVisual deprivation causes enhanced eye growth and the development of myopia, which is associated with a change in the arrangement of collagen fibers within the sclera. A second harmonic generation (SHG) microscope has been used to image the collagen fibers of unstained scleral punches from the posterior part of chicken eyes. We aimed to analyze the fibrous scleral tissue and quantify the changes in collagen organization in relation to the extent of induced deprivation myopia. The scleral architecture was assessed with the Radon transform (RT) through the parameter called structural dispersion (SD) that provides an objective tool to quantify the level of organization of the collagen network. We found that final refraction and axial length changes were linearly correlated. However, no significant differences in scleral thickness were found for different amounts of induced myopia. In contrast, a significant correlation between SD and refraction was demonstrated, ranging from a non-organized (in the control sclerae) to a quasi-aligned distribution (with a dominant direction of the fibers, in the sclera of myopic chicks). These findings demonstrate a remodeling process of the scleral collagen associated with myopia progression that can be measured accurately combining SHG imaging microscopy and RT algorithms.
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