Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10201/49309
Twittear
Registro completo de metadatos
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Ohno, Jun | - |
dc.contributor.author | Iwahashi, Teruaki | - |
dc.contributor.author | Ehara, Michiko | - |
dc.contributor.author | Ozasa, Ryuki | - |
dc.contributor.author | Hanada, Hironori | - |
dc.contributor.author | Funakoshi, Tomoyuki | - |
dc.contributor.author | Taniguchi, Kunihisa | - |
dc.date.accessioned | 2016-04-25T11:11:56Z | - |
dc.date.available | 2016-04-25T11:11:56Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | http://hdl.handle.net/10201/49309 | - |
dc.description.abstract | To elucidate the involvement of intercellular adhesion molecule-1 (ICAM-1) in the migration of lymphocytes to the oral mucosal epithelium in a rat model of acute graft-versus-host disease (AGVHD), we investigated (1) ICAM-1 and major histocompatibility complex (MHC) class II expression by keratinocytes (KCs) and their role in the epithelial infiltration of CD8+ cells, (2) the tissue expression of interferon-γ (IFN-γ) mRNA and expression of IFN-γ receptor by KCs, and (3) the ability of KCs to direct CD8+ cells into the epithelial layers. We classified the oral mucosal lesions into three consecutive temporal phases on the basis of increased epithelial ICAM-1expression: basal- (phase I), parabasal- (phase II), and pan-epithelial except for the cornified cell layer (phase III). Basal ICAM-1 expression by KCs preceded that of MHC class II molecules, infiltration of CD8+ cells and epithelial histological changes. Tissue expression of IFN-γ mRNA and expression of IFN-γ receptor on KCs evidenced by immunohistochemistry were detected in early lesions (phase I), indicating that locally produced IFN-γ induced ICAM-1 expression by KCs. CD8+ cells were bound to KCs in frozen sections of epithelial lesions, whereas no lymphocyte attachment was observed in normal KC. Adherence could be inhibited by pretreating CD8+ cells with lymphocyte function-associated antigen-1 (LFA-1) antibody and/or by pretreating sections with ICAM-1 antibody. Our data suggest that in the early phase of acute oral mucosal GVHD, the induction of ICAM-1 expression on KCs leads to the migration of CD8+ cells into the epithelium and that this is mediated in part by the ICAM-1/LFA-1 pathway. | es |
dc.format | application/pdf | es |
dc.format.extent | 9 | es |
dc.language | eng | es |
dc.publisher | Editores F. Hernandez y Juan F. Madrid. Murcia, Universidad de Murcia, Departamento de Biologia Celular e Histologiam | es |
dc.relation.ispartof | Histology and Histopathology, vol.26, nº6, 2011 | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | ICAM-1 | es |
dc.subject | GVHD | es |
dc.subject | Oral mucosa | es |
dc.subject.other | 61 - Medicina | es |
dc.title | Induction of epithelial migration of lymphocytes by intercellular adhesion molecule-1 in a rat model of oral mucosal graft-versus-host disease | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.26, nº6 (2011) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Ohno-26-725-733-2011.pdf | 1,18 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons