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Título: | Matrix metalloproteinases and their role in the renal epithelial mesenchymal transition |
Fecha de publicación: | 2011 |
Editorial: | Murcia: F. Hernández |
ISSN: | 1699-5848 0213-3911 |
Materias relacionadas: | 636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos |
Palabras clave: | Fibrosis Zymography |
Resumen: | Tubular cell epithelial-mesenchymal transition (EMT) is a fundamental contributor to renal fibrosis. The aim of this study was to investigate the activity of different matrix metalloproteinases by immunohistochemistry and gel-zymography in a model of chronic canine kidney disease. Immunohistochemistry for antibodies against MMP-9, MMP-2, MMP-13, MMP-14 and TIMP-2 was performed on 28 renal biopsy specimens. Selected cases were chosen for gelatin zymography. In moderate and severe tubulo-interstitial damage, increased expression of MMP-2 was noted. A peculiar staining pattern for MMP-2 in variable-sized vesicles, corresponding to the area of basement membrane splitting, was observed. The immunoexpression of MMP-9 and TIMP-2 was reduced in the same cases, compared to control dogs. The splitting of the membrane suggests an active role of this gelatinase in the disruption of type-IV collagen, the main basement membrane component, confirmed by MMP2 gelatinolytic activity by gel-zymography. These data could provide the basis for clinical trials examining the potential benefits of selective MMP-2 inhibitors in dogs with chronic kidney disease. |
Autor/es principal/es: | Aresu, L. Benali, S. Garbisa, S. Gallo, E. Castagnaro, M. |
Forma parte de: | Histology and histopathology, Vol. 26, nº 3 (2011) |
URI: | http://hdl.handle.net/10201/48275 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 7 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.26, nº3 (2011) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Aresu-26-307-313-2011.pdf | 1,73 MB | Adobe PDF | Visualizar/Abrir |
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