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http://hdl.handle.net/10201/46603
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Título: | Sudan Black B treatment reduces autofluorescence and improves resolution of in situ hybridization specific fluorescent signals of brain sections |
Fecha de publicación: | 2010 |
Editorial: | Murcia: F. Hernández |
ISSN: | 1699-5848 0213-3911 |
Materias relacionadas: | 616 - Patología. Medicina clínica. Oncología |
Palabras clave: | Human brain Autofluorescence |
Resumen: | Interference by autofluorescence is one of the major concerns of immunofluorescence analysis of in situ hybridization-based diagnostic assays. We present a useful technique that reduces autofluorescent background without affecting the tissue integrity or direct immunofluorescence signals in brain sections. Using six different protocols, such as ammonia/ethanol, Sudan Black B (SBB) in 70% ethanol, photobleaching with UV light and different combinations of them in both formalin-fixed paraffin-embedded and frozen human brain tissue sections, we have found that tissue treatment of SBB in a concentration of 0.1% in 70% ethanol is the best approach to reduce/eliminate tissue autofluorescence and background, while preserving the specific fluorescence hybridization signals. This strategy is a feasible, non-time consuming method that provides a reasonable compromise between total reduction of the tissue autofluorescence and maintenance of specific fluorescent labels. |
Autor/es principal/es: | Oliveira, V.C. Carrara, R.C.V. Simoes, D.L.C. Saggioro, F.P. Carlotti, C.G. Covas, D.T., Jr. Neder, L. |
Forma parte de: | Histology and histopathology, Vol. 25, nº 8 (2010) |
URI: | http://hdl.handle.net/10201/46603 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 8 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.25, nº8 (2010) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Sudan Black B treatment reduces autofluorescence.pdf | 1,76 MB | Adobe PDF | ![]() Visualizar/Abrir |
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