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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Oliveira, V.C. | - |
dc.contributor.author | Carrara, R.C.V. | - |
dc.contributor.author | Simoes, D.L.C. | - |
dc.contributor.author | Saggioro, F.P. | - |
dc.contributor.author | Carlotti, C.G. | - |
dc.contributor.author | Covas, D.T., Jr. | - |
dc.contributor.author | Neder, L. | - |
dc.date.accessioned | 2015-10-13T14:40:01Z | - |
dc.date.available | 2015-10-13T14:40:01Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1699-5848 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | http://hdl.handle.net/10201/46603 | - |
dc.description.abstract | Interference by autofluorescence is one of the major concerns of immunofluorescence analysis of in situ hybridization-based diagnostic assays. We present a useful technique that reduces autofluorescent background without affecting the tissue integrity or direct immunofluorescence signals in brain sections. Using six different protocols, such as ammonia/ethanol, Sudan Black B (SBB) in 70% ethanol, photobleaching with UV light and different combinations of them in both formalin-fixed paraffin-embedded and frozen human brain tissue sections, we have found that tissue treatment of SBB in a concentration of 0.1% in 70% ethanol is the best approach to reduce/eliminate tissue autofluorescence and background, while preserving the specific fluorescence hybridization signals. This strategy is a feasible, non-time consuming method that provides a reasonable compromise between total reduction of the tissue autofluorescence and maintenance of specific fluorescent labels. | es |
dc.format | application/pdf | es |
dc.format.extent | 8 | es |
dc.language | eng | es |
dc.publisher | Murcia: F. Hernández | es |
dc.relation.ispartof | Histology and histopathology, Vol. 25, nº 8 (2010) | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.subject | Human brain | es |
dc.subject | Autofluorescence | es |
dc.subject.other | 616 - Patología. Medicina clínica. Oncología | es |
dc.title | Sudan Black B treatment reduces autofluorescence and improves resolution of in situ hybridization specific fluorescent signals of brain sections | es |
dc.type | info:eu-repo/semantics/article | es |
Aparece en las colecciones: | Vol.25, nº8 (2010) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Sudan Black B treatment reduces autofluorescence.pdf | 1,76 MB | Adobe PDF | Visualizar/Abrir |
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