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Título: | Enhanced expression of endothelin-1 and endothelinconverting enzyme-1 in acute hypoxic rat aorta |
Fecha de publicación: | 2002 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina |
Palabras clave: | Hypoxia Rat aorta |
Resumen: | Deeply anesthetized male Wistar rats were perfused by Hanks’ balanced salt solution bubbled with either 95%air and 5%CO2 (normoxic group) or 95%N2 and 5%CO2 (hypoxic group) from the thoracic aorta for 30 min, and the isolated abdominal aortae from both groups were used for electron microscopy, immunocytochemistry of endothelin (ET)-1 and ETconverting enzyme (ECE)-1, and in situ hybridization of p r e p r o E T-1 mRNA. A remarkable increase in the number of Weibel-Palade (WP) bodies, storage sites of E T-1 and ECE-1, occurred in the hypoxic group when compared to the normoxic group. Immunoreactivities for E T-1 and ECE-1, and signals for preproET-1 mRNA were seen along the endothelia of both groups, but the intensities were significantly elevated in the hypoxic group. The increase in the number of ECE-1 immunoreactive gold particles was noticed especially in WP bodies in the hypoxic group. These findings indicate the enhancement of preproET-1 synthesis in the aortic endothelial cells as well as the acceleration of ET- 1 processing in increased WP bodies in such cells in an experimentally hypoxic condition of the rat aortae. |
Autor/es principal/es: | Doi, Y. Kudo, H. Nishino, T. Yamamoto, O. Nagata, T. Nara, S. Morita, M. Fujimoto, S. |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/20764 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 9 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol.17, nº 1 (2002) |
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Enhanced expression of endothelin1 and endothelinconverting.pdf | 2,23 MB | Adobe PDF | Visualizar/Abrir |
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