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Título: | HBsAg, HBcAg and Delta-Ag in liver tissue: simultaneous visualization in a single tissue section by triple immunostaining |
Fecha de publicación: | 1986 |
Editorial: | Murcia : F. Hernández |
ISSN: | 0213-3911 |
Materias relacionadas: | CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología |
Palabras clave: | Viral hepatitis Immunohistochemistry |
Resumen: | The distribution pattern of HBsAg, HBcAg and Delta-Ag was investigated by immunohistochemistry in a series of paraffin embedded liver tissue specimens from 45 subjects with serum HBsAg and anti-Delta antibody positivity. An indirect immunoperoxidase technique was used. Stains for HBsAg, HBcAg and Delta-Ag were alternatively carried out on serial tissue sections and, for the first time, consecutively in a single section (triple immunostaining). Simultaneous presence of all antigens occurred in 7 out of 45 cases, and of two antigens (HBsAg and Delta-Ag) in the remaining 38. Two antigens (either HBsAg and HBcAg or HBcAg and Delta-Ag) could also be shown in the same cell. A series of new observations was forwarded by the successful application of triple immunostaining in the present series: 1) high frequence of simultaneous presence of HBcAg and Delta-Ag (7 out of 45 cases = 16%); 2) cytoplasmic localization of Delta-Ag; 3) localization of HBcAg on the cell membrane of HBsAg positive Ground- Glass (G-G) hepatocytes; 4) Ground-Glass appearance of hepatocytic cytoplasm associated with exclusive content of HBcAg (HBcAg-Ground-Glass: a new variant of G-G-hepatocytes). |
Autor/es principal/es: | Facchetti, Fabio Tardanico, Regina Bonetti, Maria F. Guerini, Agostino Callea, Francesco |
Forma parte de: | Histology and histopathology |
URI: | http://hdl.handle.net/10201/17812 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 5 |
Derechos: | info:eu-repo/semantics/openAccess |
Aparece en las colecciones: | Vol. 1, nº 2 (1986) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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HBsAg HBcAg and DeltaAg in liver tissue simultaneous visualization in a single tissue section by triple immunostaining1.pdf | 334,64 kB | Adobe PDF | Visualizar/Abrir |
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