Please use this identifier to cite or link to this item: http://hdl.handle.net/10201/17812

Title: HBsAg, HBcAg and Delta-Ag in liver tissue: simultaneous visualization in a single tissue section by triple immunostaining
Issue Date: 1986
Publisher: Murcia : F. Hernández
ISSN: 0213-3911
Related subjects: CDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología
Keywords: Viral hepatitis
Immunohistochemistry
Abstract: The distribution pattern of HBsAg, HBcAg and Delta-Ag was investigated by immunohistochemistry in a series of paraffin embedded liver tissue specimens from 45 subjects with serum HBsAg and anti-Delta antibody positivity. An indirect immunoperoxidase technique was used. Stains for HBsAg, HBcAg and Delta-Ag were alternatively carried out on serial tissue sections and, for the first time, consecutively in a single section (triple immunostaining). Simultaneous presence of all antigens occurred in 7 out of 45 cases, and of two antigens (HBsAg and Delta-Ag) in the remaining 38. Two antigens (either HBsAg and HBcAg or HBcAg and Delta-Ag) could also be shown in the same cell. A series of new observations was forwarded by the successful application of triple immunostaining in the present series: 1) high frequence of simultaneous presence of HBcAg and Delta-Ag (7 out of 45 cases = 16%); 2) cytoplasmic localization of Delta-Ag; 3) localization of HBcAg on the cell membrane of HBsAg positive Ground- Glass (G-G) hepatocytes; 4) Ground-Glass appearance of hepatocytic cytoplasm associated with exclusive content of HBcAg (HBcAg-Ground-Glass: a new variant of G-G-hepatocytes).
Primary author: Facchetti, Fabio
Tardanico, Regina
Bonetti, Maria F.
Guerini, Agostino
Callea, Francesco
Published in: Histology and histopathology
URI: http://hdl.handle.net/10201/17812
Document type: info:eu-repo/semantics/article
Number of pages / Extensions: 5
Rights: info:eu-repo/semantics/openAccess
Appears in Collections:Vol. 1, nº 2 (1986)



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