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Título: | The transcriptome of pig spermatozoa, and its role in fertility |
Fecha de publicación: | 25-feb-2020 |
Editorial: | MDPI |
Cita bibliográfica: | Int. J. Mol. Sci. 2020, 21, 1572 |
ISSN: | Print: 1661-6596 Electronic: 1422-0067 |
Palabras clave: | Transcriptomics Microarrays Bioinformatics Spermatozoa Fertility Pig |
Resumen: | In the study presented here we identified transcriptomic markers for fertility in the cargo of pig ejaculated spermatozoa using porcine-specific micro-arrays (GeneChip® miRNA 4.0 and GeneChip® Porcine Gene 1.0 ST). We report (i) the relative abundance of the ssc-miR-1285, miR-16, miR-4332, miR-92a, miR-671-5p, miR-4334-5p, miR-425-5p, miR-191, miR-92b-5p and miR-15b miRNAs, and (ii) the presence of 347 up-regulated and 174 down-regulated RNA transcripts in high-fertility breeding boars, based on differences of farrowing rate (FS) and litter size (LS), relative to low-fertility boars in the (Artificial Insemination) AI program. An overrepresentation analysis of the protein class (PANTHER) identified significant fold-increases for C-C chemokine binding (GO:0019957): CCR7, which activates B- and T-lymphocytes, 8-fold increase), XCR1 and CXCR4 (with ubiquitin as a natural ligand, 1.24-fold increase), cytokine receptor activity (GO:0005126): IL23R receptor of the IL23 protein, associated to JAK2 and STAT3, 3.4-fold increase), the TGF-receptor (PC00035) genes ACVR1C and ACVR2B (12-fold increase). Moreover, two micro-RNAs (miR-221 and mir-621) were down- and up-regulated, respectively, in high-fertility males. In conclusion, boars with different fertility performance possess a wide variety of differentially expressed RNA present in spermatozoa that would be attractive targets as non-invasive molecular markers for predicting fertility. |
Autor/es principal/es: | Álvarez Rodríguez, Manuel Martínez, Cristina Wright, Dominic Barranco, Isabel Roca, Jordi Rodríguez Martínez, Heriberto |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Medicina y Cirugía Animal |
Versión del editor: | https://www.mdpi.com/1422-0067/21/5/1572 |
URI: | http://hdl.handle.net/10201/143679 |
DOI: | https://doi.org/10.3390/ijms21051572 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 23 |
Derechos: | info:eu-repo/semantics/openAccess Atribución 4.0 Internacional |
Descripción: | © 2020 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in International Journal of Molecular Sciences. To access the final edited and published work see https://doi.org/10.3390/ijms21051572 |
Aparece en las colecciones: | Artículos: Medicina y Cirugía Animal |
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