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https://doi.org/10.1111/j.1365-2362.2010.02368.x
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Título: | Peritoneal macrophage priming in cirrhosis is related to ERK phosphorylation and IL-6 secretion. |
Fecha de publicación: | 19-ago-2010 |
Editorial: | Wiley |
Cita bibliográfica: | European Journal of Clinical Investigation. 2011, Vol. 41 (1), pp. 8-15 |
ISSN: | Print: 0014-2972 Electronic: 1365-2362 |
Palabras clave: | Ascitic fluid Cirrhosis Cytokines Macrophages MAP kinases |
Resumen: | Background: Bacterial infections are common complications arising in patients with cirrhosis and ascites. Translocation of bacterial DNA is a dynamic process that is associated with an increased inflammatory response and a poor prognosis in this setting. The aim of this study was to study whether peritoneal macrophages remain in a chronic primed status to allow a rapid response to subsequent events of bacterial translocation. Patients and methods: Peritoneal monocyte-derived macrophages were isolated from 25 patients with cirrhosis and non-infected ascites and compared with donor's blood monocytes. Activation cell-surface markers were screened using flow-cytometry, and the phosphorylation state of ERK 1/2, p38 MAP Kinase, PKB/Akt and transcription factors c-Jun and p65 NFκB were evaluated using Western blot. Synthesis of tumour necrosis factor alpha, interleukin 6 (IL-6) and interleukin-10 (IL-10) at baseline and in response to bacterial stimuli was evaluated using ELISA. Results: A high expression of CD54, CD86 and HLA-DR at baseline was displayed by peritoneal macrophages. Increased phosphorylated levels of ERK1/2, protein kinase B (PKB) and c-Jun, together with IL-6 production, were observed in peritoneal macrophages at baseline compared with donors' blood monocytes. A positive correlation was established between basal IL-6 levels and extracellular signal-regulated kinase (ERK) phosphorylation in peritoneal macrophages from patients with cirrhosis (r=0·9; P=0·005). Addition of lipopolysaccharide induced higher phosphorylation levels of all studied signalling intermediates than synthetic-oligodeoxydinucleotides, but similar end-stage p65 NFκB. Conclusions: A sustained immune response is present in ascitic fluid of cirrhotic patients, even in the temporal absence of bacterial antigens. This would facilitate a fast response, probably controlled by IL-6, against repeated bacterial-DNA translocation or in liver chronic inflammation. |
Autor/es principal/es: | Ruiz Alcaraz, Antonio J. Martínez Esparza, M. Caño, Rocío Hernández Caselles, Trinidad Ricarti, Chiara Llanos, Lucía Zapater, Pedro Tapia Abellán, Ana Martín Orozco, Elena Pérez Mateo, Miguel Such, José García Peñarrubia, Pilar Francés, Rubén |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología |
Versión del editor: | https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2362.2010.02368.x |
URI: | http://hdl.handle.net/10201/142823 |
DOI: | https://doi.org/10.1111/j.1365-2362.2010.02368.x |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 8 |
Derechos: | info:eu-repo/semantics/embargoedAccess |
Descripción: | © 2010 The Authors. This document is the Published version of a Published Work that appeared in final form in European Journal of Clinical Investigation. To access the final edited and published work see https://doi.org/10.1111/j.1365-2362.2010.02368.x |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
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Fichero | Descripción | Tamaño | Formato | |
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EJCI_cirrosis_2011.pdf | 266,06 kB | Adobe PDF | ![]() Visualizar/Abrir Solicitar una copia |
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