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https://doi.org/10.1111/j.1365-2362.2010.02368.x
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Ruiz Alcaraz, Antonio J. | - |
dc.contributor.author | Martínez Esparza, M. | - |
dc.contributor.author | Caño, Rocío | - |
dc.contributor.author | Hernández Caselles, Trinidad | - |
dc.contributor.author | Ricarti, Chiara | - |
dc.contributor.author | Llanos, Lucía | - |
dc.contributor.author | Zapater, Pedro | - |
dc.contributor.author | Tapia Abellán, Ana | - |
dc.contributor.author | Martín Orozco, Elena | - |
dc.contributor.author | Pérez Mateo, Miguel | - |
dc.contributor.author | Such, José | - |
dc.contributor.author | García Peñarrubia, Pilar | - |
dc.contributor.author | Francés, Rubén | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular B e Inmunología | es |
dc.date.accessioned | 2024-07-03T08:02:06Z | - |
dc.date.available | 2024-07-03T08:02:06Z | - |
dc.date.issued | 2010-08-19 | - |
dc.identifier.citation | European Journal of Clinical Investigation. 2011, Vol. 41 (1), pp. 8-15 | es |
dc.identifier.issn | Print: 0014-2972 | - |
dc.identifier.issn | Electronic: 1365-2362 | - |
dc.identifier.uri | http://hdl.handle.net/10201/142823 | - |
dc.description | © 2010 The Authors. This document is the Published version of a Published Work that appeared in final form in European Journal of Clinical Investigation. To access the final edited and published work see https://doi.org/10.1111/j.1365-2362.2010.02368.x | - |
dc.description.abstract | Background: Bacterial infections are common complications arising in patients with cirrhosis and ascites. Translocation of bacterial DNA is a dynamic process that is associated with an increased inflammatory response and a poor prognosis in this setting. The aim of this study was to study whether peritoneal macrophages remain in a chronic primed status to allow a rapid response to subsequent events of bacterial translocation. Patients and methods: Peritoneal monocyte-derived macrophages were isolated from 25 patients with cirrhosis and non-infected ascites and compared with donor's blood monocytes. Activation cell-surface markers were screened using flow-cytometry, and the phosphorylation state of ERK 1/2, p38 MAP Kinase, PKB/Akt and transcription factors c-Jun and p65 NFκB were evaluated using Western blot. Synthesis of tumour necrosis factor alpha, interleukin 6 (IL-6) and interleukin-10 (IL-10) at baseline and in response to bacterial stimuli was evaluated using ELISA. Results: A high expression of CD54, CD86 and HLA-DR at baseline was displayed by peritoneal macrophages. Increased phosphorylated levels of ERK1/2, protein kinase B (PKB) and c-Jun, together with IL-6 production, were observed in peritoneal macrophages at baseline compared with donors' blood monocytes. A positive correlation was established between basal IL-6 levels and extracellular signal-regulated kinase (ERK) phosphorylation in peritoneal macrophages from patients with cirrhosis (r=0·9; P=0·005). Addition of lipopolysaccharide induced higher phosphorylation levels of all studied signalling intermediates than synthetic-oligodeoxydinucleotides, but similar end-stage p65 NFκB. Conclusions: A sustained immune response is present in ascitic fluid of cirrhotic patients, even in the temporal absence of bacterial antigens. This would facilitate a fast response, probably controlled by IL-6, against repeated bacterial-DNA translocation or in liver chronic inflammation. | es |
dc.format | application/pdf | es |
dc.format.extent | 8 | es |
dc.language | eng | es |
dc.publisher | Wiley | es |
dc.relation | This study was funded by grants PI06 ⁄ 1453, PI06 ⁄ 0006 and CP05 ⁄ 0005 from Instituto de Salud Carlos III, Madrid, Spain and 11926 ⁄ PI ⁄ 09 from Fundación Séneca (CARM, Murcia, Spain). Ana Tapia-Abellán has a PhD fellowship from Fundación Séneca (12302/FPI/09). | es |
dc.rights | info:eu-repo/semantics/embargoedAccess | es |
dc.subject | Ascitic fluid | es |
dc.subject | Cirrhosis | es |
dc.subject | Cytokines | es |
dc.subject | Macrophages | es |
dc.subject | MAP kinases | es |
dc.title | Peritoneal macrophage priming in cirrhosis is related to ERK phosphorylation and IL-6 secretion. | es |
dc.type | info:eu-repo/semantics/article | es |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2362.2010.02368.x | es |
dc.embargo.terms | SI | - |
dc.identifier.doi | https://doi.org/10.1111/j.1365-2362.2010.02368.x | - |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
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Fichero | Descripción | Tamaño | Formato | |
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EJCI_cirrosis_2011.pdf | 266,06 kB | Adobe PDF | ![]() Visualizar/Abrir Solicitar una copia |
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