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Título: | Development and characterization of 15 novel polymorphic microsatellite loci for two important bot flies (Diptera, Oestridae) by next-generation sequencing |
Fecha de publicación: | 2020 |
Editorial: | Springer |
Cita bibliográfica: | Parasitology Research (2020) 119:2829–2835 |
ISSN: | Print: 0932-0113 Electronic: 1432-1955 |
Palabras clave: | Oestrus ovis Cephenemyia stimulator Microsatellites Next-generation sequencing |
Resumen: | Cephenemyia stimulator and Oestrus ovis are two important parasitic bot flies (Oestridae) species causing myiasis, with a potential negative impact on the welfare of the host. Using next-generation sequencing approach and bioinformatics tools, a large panel of possible microsatellites loci was obtained in both species. Primer pairs were designed for 15 selected microsatellite loci in C. stimulator and other 15 loci in O. ovis for PCR amplification. Loci amplification and analysis were performed in four populations of each species. The results demonstrated that all selected loci were polymorphic, with the number of alleles ranging from 2 to 6 per locus in C. stimulator and 3 to 13 per locus in O. ovis. This is the first time to describe these microsatellite loci for C. stimulator and O. ovis. These two sets of microsatellite markers could be further used for biogeographic and population genetics studies. Cephenemyia stimulator and Oestrus ovis, are two important parasitic bot flies (Oestridae) species causing myiasis, with a potential negative impact on the welfare of the host. Using next-generation sequencing approach and bioinformatics tools a large panel of possible microsatellites loci was obtained in both species. Primer pairs were designed for 15 selected microsatellite loci in C. stimulator and other 15 loci in O. ovis for PCR amplification. Loci amplification and analysis were performed in four populations of each species. The results demonstrated that all selected loci were polymorphic, with the number of alleles ranging from 2 to 6 per locus in C. stimulator and 3 to 13 per locus in O. ovis. This is the first time to describe these microsatellite loci for C. stimulator and O. ovis. These two sets of microsatellite markers could be further used for biogeographic and population genetics studies. |
Autor/es principal/es: | Aleix-Mata, Gaël Expósito, Miriam Ruiz Ruano, Francisco J. López Beceiro, Ana María Fidalgo, Luis E Martínez Carrasco-Pleite, Carlos Ruiz de Ybáñez Carnero, María del Rocío Boos, Mathieu Pérez, Jesús M. Sánchez, Antonio |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Facultad de Veterinaria |
URI: | http://hdl.handle.net/10201/139052 |
DOI: | https://doi.org/10.1007/s00436-020-06824-y |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 18 |
Derechos: | info:eu-repo/semantics/openAccess |
Descripción: | ©2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted, version of a Published Work that appeared in final form in Parasitology Research. To access the final edited and published work see https://doi.org/10.1007/s00436-020-06824-y |
Aparece en las colecciones: | Artículos: Sanidad Animal |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
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Microsatélites bot flies_ versión aceptada.pdf | 331,15 kB | Adobe PDF | Visualizar/Abrir |
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