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https://doi.org/10.1007/s00436-020-06824-y
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Aleix-Mata, Gaël | - |
dc.contributor.author | Expósito, Miriam | - |
dc.contributor.author | Ruiz Ruano, Francisco J. | - |
dc.contributor.author | López Beceiro, Ana María | - |
dc.contributor.author | Fidalgo, Luis E | - |
dc.contributor.author | Martínez Carrasco-Pleite, Carlos | - |
dc.contributor.author | Ruiz de Ybáñez Carnero, María del Rocío | - |
dc.contributor.author | Boos, Mathieu | - |
dc.contributor.author | Pérez, Jesús M. | - |
dc.contributor.author | Sánchez, Antonio | - |
dc.contributor.other | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Facultad de Veterinaria | es |
dc.date.accessioned | 2024-02-09T08:29:57Z | - |
dc.date.available | 2024-02-09T08:29:57Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Parasitology Research (2020) 119:2829–2835 | es |
dc.identifier.issn | Print: 0932-0113 Electronic: 1432-1955 | - |
dc.identifier.uri | http://hdl.handle.net/10201/139052 | - |
dc.description | ©2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted, version of a Published Work that appeared in final form in Parasitology Research. To access the final edited and published work see https://doi.org/10.1007/s00436-020-06824-y | - |
dc.description.abstract | Cephenemyia stimulator and Oestrus ovis are two important parasitic bot flies (Oestridae) species causing myiasis, with a potential negative impact on the welfare of the host. Using next-generation sequencing approach and bioinformatics tools, a large panel of possible microsatellites loci was obtained in both species. Primer pairs were designed for 15 selected microsatellite loci in C. stimulator and other 15 loci in O. ovis for PCR amplification. Loci amplification and analysis were performed in four populations of each species. The results demonstrated that all selected loci were polymorphic, with the number of alleles ranging from 2 to 6 per locus in C. stimulator and 3 to 13 per locus in O. ovis. This is the first time to describe these microsatellite loci for C. stimulator and O. ovis. These two sets of microsatellite markers could be further used for biogeographic and population genetics studies. | es |
dc.description.abstract | Cephenemyia stimulator and Oestrus ovis, are two important parasitic bot flies (Oestridae) species causing myiasis, with a potential negative impact on the welfare of the host. Using next-generation sequencing approach and bioinformatics tools a large panel of possible microsatellites loci was obtained in both species. Primer pairs were designed for 15 selected microsatellite loci in C. stimulator and other 15 loci in O. ovis for PCR amplification. Loci amplification and analysis were performed in four populations of each species. The results demonstrated that all selected loci were polymorphic, with the number of alleles ranging from 2 to 6 per locus in C. stimulator and 3 to 13 per locus in O. ovis. This is the first time to describe these microsatellite loci for C. stimulator and O. ovis. These two sets of microsatellite markers could be further used for biogeographic and population genetics studies. | - |
dc.format | application/pdf | es |
dc.format.extent | 18 | - |
dc.language | eng | es |
dc.publisher | Springer | - |
dc.relation | Fédération Nationale des Chasseurs (France), Fédération Départementale des Chasseurs de l’Ariège and Hautes-Pyrénées (France) (project FNC-PSN-PR16-2014). Fédération Nationale des Chasseurs (France) (project: FNC-PSN-PR5-2013), National Institute for The Agriculture and Food Research and Technology (INIA), Madrid (project: FAU2008-00010-00-00) and Jaén University (project: RFC/PP2008/UJA_68_16_22). | es |
dc.rights | info:eu-repo/semantics/openAccess | es |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Oestrus ovis | es |
dc.subject | Cephenemyia stimulator | es |
dc.subject | Microsatellites | es |
dc.subject | Next-generation sequencing | es |
dc.title | Development and characterization of 15 novel polymorphic microsatellite loci for two important bot flies (Diptera, Oestridae) by next-generation sequencing | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | https://doi.org/10.1007/s00436-020-06824-y | - |
Aparece en las colecciones: | Artículos: Sanidad Animal |
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Microsatélites bot flies_ versión aceptada.pdf | 331,15 kB | Adobe PDF | ![]() Visualizar/Abrir |
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