Histology and histopathology Vol. 8, nº 1 (1993)
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- PublicationOpen AccessClinico-pathological correlations in meningiomas, a DNA and immunohistochemical study(Murcia : F. Hernández, 1993) Cruz-Sánchez, F. F.; Miquel, R.; Rossi, M.L.; Figols, J.; Palacín, A.; Cardesa, AntonioWe have studied 41 meningiomas classified histologically as benjgn, atypical or anaplastic. There were 26 females and 15 males and the mean age was 53 years. 36 tumours were supratentorial, 4 infratentorial and one spinal. Flow cytometry was performed on paraffin-embedded tissue using a selective staining technique for DNA. The ploidy index of DNA and percentage of cells in the S and G2/M phases were calculated. Results were correlated with clinical, histological and immunohistological data. 16/41 tumours . were found to be diploid, 17/41 aneuploid and 8/41 could not be analysed. Significant correlations were found between aneuploid tumours and some qualitative features such as recurrence, pleomorphism, high cellular density, mitotic activity and brain and soft tissue infiltration. A high proliferative index appeared to be associated with clinical aggressiveness. No particular correlation between the expression of cytokeratin and epithelial membrane antigen markers and flow cytometry was found. Our results suggest that DNA flow cytometry in meningiomas may be of value in predicting the behaviour of these neoplasms and confirm that epithelial pattern in meningiomas is not linked to increased anaplasia or poor prognosis.
- PublicationOpen AccessInhibition of gentamicin-induced nephrotoxicity by lithium in rat(Murcia : F. Hernández, 1993) Samadian, T.; Dehpour, A.R.; Amini, Sh.; Nouhnejad, P.Daily intraperitoneal injection of gentamicin in doses of 2,4 and 10 mglkglday for 5 consecutive days produced proximal tubular necrosis in male albino rats as assessed by ultrastructural findings from electron microscopic observations. With respect to nephrotoxicity, aminoglycoside antibiotics (AGs) have been shown to concentrate in the lysosomes of kidney proximal tubular cells to inhibit the activities of phospholipases A and C, including a phospholipidosis, characterized by the formation of myeloid bodies. It has been suggested that the nephrotoxicity of AGs is related to the extent of this phospholipidosis. The concurrent therapy of lithium in doses of 5 and 10 mEqIkglday, administered subcutaneously, 24 hours prior to gentamicin administration for the same period, proved effective in reducing the gentamicin-induced phospholipidosis in kidney as judged by reduction in lysosomal myeloid bodies to an amount of 26-45 percent. It is well known that lithium interferes with phosphatidylinositol turnover and reduces the cellular availability of myoinositol which is needed for the resynthesis of membrane poliphosphoinositides. Thus, the inhibitory effect of lithium on gentamicin-induced nephrotoxicity may be due to interference of lithium with phosphoinositide cycle.
- PublicationOpen AccessEpidermoid carcinoma of the lung in stage I, Factors of prognostic interest(Murcia : F. Hernández, 1993) Carbajo, M.; Ortega, F.J.; Hernández, M.; Ondiviela, R.; Blanco, C.; Buelta, L.; Garijo, F.; Val-Bernal, F.Factors affecting stage 1 epidermoid cancer of the lung were studied in a series of 29 patients treated only by surgery and followed up for ten years. A set of 13 variables with a possible influence on prognosis were investigated. The application of the Cox Univariate Analysis to the different variables showed the grade of cell differentiation and the mitotic index to be predictors. In the Cox Multivariate Analysis, the proportional regression equation revealed two independently significant variables (p<0.01), which were the Mitotic Index and Nuclear Area. Grouping patients on the basis of the prognostic variables indicated allows a better prediction for survival to be made for this series of patients.
- PublicationOpen AccessUltrastructural substrates for increased lung water content in experimental pulmonary edema(Murcia : F. Hernández, 1993) Oda, H.; Arakawa, M.; Kambara, K.; Nakahara, K.; Segawa, T.; Ando, F.; Kawada, T.; Hirakawa, S.; Shoumura, S.; Isono, H.We examined the relationship between the incidence of ultrastructural changes in the alveolar septum and the extravascular lung water content. Pulmonary edema was induced in 18 mongrel dogs by either dextran (n=12) or alloxan (n=6) administration. Six other dogs served as controls. Extravascular lung water content was measured by the thermal-dye double indicator dilution method. Specimens of lung tissue were examined with an electron microscope, and the incidence of 13 types of pathological changes in the alveolar septum was studied. For each type of pathological change, the incidence was correlated with the magnitude of lung water content. The following results were obtained. The incidence of edematous changes in the alveolar interstitium (widening of the interstitial space, and dispersion and disarray of collagen fibres in the interstitial space) was well correlated with lung water content (r=0.78, p<0.01, and r=0.84, pc0.0 1, respectively). The correlation was not significant in the remaining types of changes. We conclude that the incidence of the pathological changes in the alveolar septum is increased along with the increase in the content of lung water in both dextran- and alloxaninduced experimental pulmonary edema in dogs.
- PublicationOpen AccessUltrastructure of granulosa lutein cells from rats fed hexachlorobenzene(Murcia : F. Hernández, 1993) MacPhee, I.J.; Singh, Amreek; Wright, Glenda M.; Foster, W.G.; LeBlanc, N.N.Corpora lutea from Sprague-Dawley rats that were orally administered 0.0 (control), 1.0, 10.0, and 100.0 mglkg hexachlorobenzene (HCB) for 21 days were analyzed by electron microscopy. Granulosa lutein cells (GLC) from animals of the 10.0 mg group showed differences from the cells of animals that served as the controls. Golgi complexes and smooth endoplasmic reticulum appeared more conspicuous, possibly due to dilation resulting from hyperactivity. Free polysomes seemed more prominent in the cells of the 10.0 mg group. The GLC architecture from animals of the 1.0 and 100.0 mg groups was similar to that of the corresponding cells in the control group. Since smooth endoplasmic reticulum is involved in the synthesis of steroid hormones, and that free polysomes are engaged in synthesis of cytoplasmic proteins, it is suggested that HCB at a dose of 10.0 mglkg given for 21 days may alter the synthetic activity of the GLC of the rat.