Histology and histopathology Vol. 9, nº 2 (1994)
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- PublicationOpen AccessThe fate of the luminal asymmetric unit membrane of the superficial cell of the rat transitional epithelium(Murcia: F. Hernández, 1994) Zhang, S.X.; Seguchi, H.The fate of the luminal asymmetric unit membrane (AUM) of the superficial cell of the transitional epithelium of the rat urinary bladder was electron microscopically and cytochemically investigated using exogenously administered horseradish peroxidase (HRP) as tracer. HRP-positive discoidal vesicles were formed by the folding of the AUM of the luminal surface plasma membrane. With the passage of time, these vesicles changed their shapes and were finally transformed into lysosomes by the following possible routes: 1) by becoming multivesicular bodies (MVBs); 2) by directly fusing with lysosomes; and 3) by becoming autophagic vacuoles. Another possibility would be reutilization.
- PublicationOpen AccessNeutrophil interaction with tumour cells in small early gastric cancer, ultrastructural observations(Murcia : F. Hernández, 1994) Caruso, R.A.; Speciale, G.; Inferrera, C.The authors studied the neutrophil inflammatory reaction by light and electron microscopy in 4 small early gastric cancers (three tubular adenocarcinomas and one adenocarcinoma with signet-ring cell component), selected for the absence of coagulative necrosis and ulceration. Neutrophils showed ultrastructural signs of activation such as aggregation, adhesion and lipid bodies. Some neutrophils were found to be in intimate contact with the intact tumour cells and with those that displayed a varying degree of damage. In particular, relatively early damage, such as disorganization of the intermediate filaments and dilatation of the rough endoplasmic reticulum, occurred at the contact regions. In spite of the severe damage to the tumour cells, the neutrophils themselves were intact. These findings are discussed in relationship to a number of recent studies of the neutrophil-mediated tumour cell injury, in man and animals.
- PublicationOpen AccessTransitional papillary cell carcinoma of the ureter, a histological and ultrastructural study(Murcia : F. Hernández, 1994) Melis, M.; Petrozza, V.; Pulvirenti, S.; Eleuteri, E.; Mosca, A.; Angelini, L.; Carpino, F.We report a case of transitional papillary carcinoma of the distal left ureter. The histological and ultrastructural features were seen and compared by SEM and TEM. Observations confirm the data of literature that the deep cells of neoplasm are a normal morphological finding while the superficial cells do not have the characteristics present in normal transitional cells. In addition, SEM shows small groups or single cells with ccpleomorphic microvilli>, on the cell surface. These, in ureter, confirm the studies of many authors who have observed these abnormalities in carcinomas of several organs like breast, colon, liver and mesothelium.
- PublicationOpen AccessThe distribution of corticotropin-releasing factor immunoreactive neurons and nerve fibres in the brain of Gambusia affinis and Salmo trutta(Murcia : F. Hernández, 1994) Coto-Montes, A.; García-Fernández, J.M.; Del Brío, M.A.; Riera, P.The study was carried out on the distribution of neurons and fibres which contain the Corticotropin releasing factor-like (CRF) immunoreactivity in the encephalon of two species of teleosts, Gambusia affinis and Salmo trutta. The peroxidase-antiperoxidase (PAP) immunocytochemical technique was employed. The present study has shown differences between both species. In Gambusia affinis, positive neurons were observed in the area ventralis telencephali pars lateralis (VLT), in the nucleus praeopticus (NPO) and in the nucleus lateralis tuberis (NLT). The immunoreactive fibres were in the area ventralis telencephali, in the preoptic hypophyseal tract and in the hypophysis. In Salmo trutta the immunoreactive cells were seen in the pars magnocellularis and pars parvocellularis of the NPO and in cerebrospinal fluid (CSF) contacting neurons of the NLT. The pattern of distribution of immunoreactive fibres in Salmo trutta was different from that in Gambusia affinis. In addition to the distribution of perikarya in Gambusia affinis (in the VLT and in the preoptic hypophyseal tract), fibres were also observed in the tubero-hypophyseal tract and in the posterior hypothalamus. The hypophysis of Salmo trutta also presents an extensive labelling. The interspecific differences shown in the present study should be due to the different degree of evolution in the two species studied and to other causes, such as environmental ones.
- PublicationOpen AccessEffects of prostaglandin Eq on the ultrastructure of the golden hamster parathyroid gland(Murcia : F. Hernández, 1994) Emura, S.; Utsumi, M.; Hayakawa, D.; Yamahira, T.; Terasawa, K.; Tamada, A.; Isono, H.; Shoumura, S.The effects of different ages on large vacuolar bodies in the parathyroid glands of golden hamsters after administration of prostaglandin E2 (PGE2) were investigated. In the parathyroid glands of the young and senile animals 15 min and the senile animals 60 min after administration of PGE2, the mean serum calcium concentration was significantly higher when compared to that of the young and senile control animals, respectively. In the experimental adult animals 60 min after administration of PGE2, the serum calcium concentration was seen to increase. In the parathyroid glands of the young animals 15 min and the adult and senile animals 60 min after administration of PGE2, the percent area occupied by large vacuolar bodies was significantly increased when compared to that of the young, adult and senile control animals, respectively. These findings suggest that the percent area occupied by large vacuolar bodies is increased in response to hypercalcemia induced by PGE2. It is thought that in the parathyroid glands suppressed by hypercalcemia there is a relationship between the percent area occupied by large vacuolar bodies and aging.