Anales de biología Vol. 10 (1986) Biología general, 2
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- PublicationOpen AccessEstudio de la autodepuración microbiológica y fisico-quimica de aguas residuales por lagunaje profundo(Murcia: Universidad de Murcia, Servicio de Publicaciones, 1986) Berná Amorós, Luis Miguel; Torrella Mateu, Francisco; Soler, A.; Sáez, J.; Llorens, M.; Martínez, I.; Facultad de BiologíaA study of the chemical and microbiological self-depuration process of urban waste-waters in a deeppond stabilization system (15000 m3 ) is presented. The pond is localized in the Campus of the University of Murcia {Spain). The study covers two independent periods of waste-water stabilization: November 1984-July 1985 and September 1985-March 1986. The pond was completely emptied between the two periods. Among the various physiological parameters studied, the following are presented in this paper: temperature, dissolved 0 2 , alkalinity, pH, and BOD5 • Microbiological analysis included total and fecal coliforms, fecal streptococci, Clostridium perfringens, and coliphages, ali of them being water pollution indicators. In the geographical area where the lagoon is located (SE Spain), the self-depuration process is relatively fast in sulface waters (0-2 m). Periods of 30 to 50 days permit a significative decrease in the organic matter content (70% BOD,) and the microbial indicators (99.9'% total and fecal coliforms, 90% C. perfringens, 100% fecal streptococci, and 85% coliphages). Nevertheless, the selfdepuration process shows a diminished rate in the deep layers of the lagoon {under 2 m) because of the envíronmental conditíons prevailing in them: low temperature and oxygen. The latter aspect if magnified in periods of water stratification, when a pronounced anaerobiosis is generated under the thermocline and the temperature remains below 15 ºC. In the area where the self-depuration system has been studied, the reutilization of the waters of deep pond systems does not present chemical or mícrobiological risks for certain uses (e.g., irrigation), if sulface waters are used after an appropriate self-depuration period. The latter depending on the organic load and the thermal regime under which the system has evolved. Keywords: Wastewater. Self-depuration. M icrobiology. Physico-chemistry.
- PublicationOpen AccessPerfil isoenzimático de hexoquinasa y enolasa en tejidos neurales(Murcia: Universidad de Murcia, Servicio de Publicaciones, 1986) Delicado, E. G.; Torres, M.; Millaruelo, A.; Miras Portugal, M. T.; Facultad de BiologíaTissues derived from the neural crest showed a characteristic distribution and activity for each glycolitic isoenzyme. The hexokinase actívity in chromaffin tissue, pheochromocytoma and melanoma was associated with the particulate fraction of the homogenate as previously described for brain hexokínase. The experimental values obtained were 1, 2.1 and 1.68 U/g respectively, corresponding to type I isoenzyme (neural isoenzyme). In spite of the hexokinase, the enolase activity ín these tissues was found in the cytosolíc fraction. The activities were 13, 25 and 110 Ulg for chromaffin tissue, pheochromocytoma and melanoma, respectively, corresponding to the r,:y hybrid form. In melanoma sorne traces of the ctct form, a non neural isoenzyme, could be detected.
- PublicationOpen AccessEffect of 2,4-Dinitrophenol, Chloramphenicol and KCN on prodigiosin production by polymyxin b treated non-proliferating cultures of Serratza marcescens(Murcia: Universidad de Murcia, Servicio de Publicaciones, 1986) El-Ebiary, A.; Guinea, J.; Viñas, M.; Lorén, J. G.; Facultad de BiologíaLa biosíntesis de prodigiosina por cultivos no proliferantes de Serratia marcescens (NPC) inducidos con L-prolina se amplifica por el efecto de la adición de algunos antibióticos. especialmente la polimixina B. El presente trabajo describe la cinética de producción de pigmento por NPC tratados con polimixína B e inducidos con L-prolina en presencia de 2,4-dinitrofenol, KCN y cloranfenícol. Los resultados demuestran que el transporte electrónico y la síntesis proteica son imprescindibles para la cromogénesis en estas condiciones, mientras que el bloqueo de la fosforilación oxidativa no evita la producción de pigmento.
- PublicationOpen AccessEstudio de los mixomicetos que fructifican sobre Opuntza fzcusindica l. en el S.E. de España peninsular(Murcia: Universidad de Murcia, Servicio de Publicaciones, 1986) López-Sánchez, E.; Honrubia García, Mario; Gracia, E.; Gea, F. J.; Facultad de BiologíaFifteen species of Myxomycetes that grow on Opuntia ficus-indica, ín South-East Spain are cited. One of them (Stemonitis virginiensis) in this substrate is collected only in moist-chamber. According to the abundante and frecuency with which the other fourteen species are registered, three groups of species are distinguished: five «characteristic» species, three «frequent» species and six «occasional» species. Badhamia gracilis is the most common one. When cultures in moist chambers were prepared sporocarps of Badhamia gracilis, Stemonitis virginiensis and Perichaena minor developed. Three species considered as «characteristic»: Badhamia gracilis, Physarum compressum and P. stramir:ipes have been cultivated in vitro. Three species widely distributed in the area studied, but that have never been collected on O. ficus-indica, were used to compare with the ones isolated in the Opuntia. Three media of culture: CM, CM/2 and carrot-agar have been used and also a new medium, prickly-agar, has been pul to test.
- PublicationOpen AccessCOMPARATIVE NEUROBIOLOGY. P. J. Mil!. Ed. Edward Arnold, Contemporary Biology. Londres, 1982, NEUROBIOLOGÍA. Gordon M. Shepherd. Ed. Labor, Barcelona, 1985.: Reseña bibliográfica(Universidad de Murcia, Servicio de Publicaciones, 1986) Costa Ruiz, Jorge de; Facultad de Biología