Anales de biología Vol. 32 (2010)

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    Somatic embryogenesis in Allium sativum L. (cv. Yamuna Safed 3): Improving embryo maturation and germination with PGRs and carbohydrates
    (Murcia : Universidad de Murcia, Servicio de Publicaciones, 2010) Abdul Nasim, Sekh; Abdul Mujib; Rashmi Kapoor; Samar Fatima; Junaid, Aslam; Mahmooduzzafar
    Callus induction was obtained from basal part of the garlic clove on Murashige and Skoog (MS) medium on which somatic embryos were formed. In Allium sativum L. cv. Yamuna Safed 3, somatic embryogeny has been categorized into three distinct stages: initiation and proliferation of embryo; maturation; and germination of embryo. The initiation of globular-stage embryo differentiation was observed on various BAP and 2,4-D concentrations but at 1.0 mg/l BAP and 0.25 mg/l 2,4-D maximum proliferation of embryos was noted. Best somatic embryo maturation was achieved on 0.5 mg/l GA3 amended medium; ABA at the same level was less effective. Embryo growth was maximum on 3% maltose while plantlet conversion was highest (65.15%) on 3% fructose added medium. Scanning electron microscopy (SEM) has been presented to demonstrate the apparent morphology and structural details of embryo. Plantlets were successfully raised from in vitro developed embryos.
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    Ultrastructure of the Outer Retina in the Killifish, Aphanius sirhani (Cyprinodontidae, Teleostei)
    (Murcia : Universidad de Murcia, Servicio de Publicaciones, 2010) Mohammad Amin Al-Adhami; Janti Qar; Muneir AlKhdour
    The ultrastructure of the outer retina in the endangered killifish, Aphanius sirhani was studied. The retina shows all eight layers and two membranes typical of vertebrates. The retinal pigment epithelium (RPE) consists of a single layer of closely packed, polygonal, low columnar cells. These cells have large, vesicular nuclei, well developed smooth endoplasmic reticulum, and well developed mitochondria. Phagosomes, lipid droplets and myeloid bodies are also evident. The cells bear numerous apical processes that, in light-adapted fish, interdigitate with the photoreceptor outer segments. The retina is of the duplex type, i.e. contains rods and cones. A variety of cones have been recorded in the present species. These include short, intermediate and long single cones, double and triangular, triple cones. Rod spherules each with a single synaptic ribbon and cone pedicles, each with several synaptic ribbons have been recorded in the outer plexiform layer.
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    Referencias bibliográficas: Syllabus der Pflanzenfamilien. Part. 3.Bryophytes and seedless vascular plants/ A. Engler’s
    (Murcia : Universidad de Murcia, Servicio de Publicaciones, 2010) Guerra Montes, Juan; Departamentos y Servicios::Departamentos de la UMU::Biología Vegetal
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    Haemosporidians in migratory blackcaps (Sylvia atricapilla), a comparison between autumn and spring periods of passage
    (Murcia : Universidad de Murcia, Servicio de Publicaciones, 2010) Arizaga, Juan; Esparza, Xavier; Barba, Emilio
    The spatio-temporal host-parasite relationships have a high interest to understand the coevolutionary processes between parasites and their hosts. Our main aim here was to analyse whether the prevalence and intensity of infection by haemoparasites in migrating blackcaps (Sylvia atricapilla), at a stopover site in N Iberia, varied throughout and between migration periods. Moreover, we studied whether the parasitaemia had any effect on fuel load. Blood smears were studied from 132 individuals. Only Haemoproteus spp. and Plasmodium spp. were detected. Trypanosoma spp., microfilaria or Leucocytozoon spp. were absent. The prevalence was 35% and it did not differ throughout and between periods or between age classes, but between the sexes. Prevalence was higher among males (45.5% versus 22.7%), suggesting that males were more prone to be infected, which could be due to sex-associated immunologic variations. The mean intensity of infection was low (2.8 parasites/2000 erythrocytes). We did not find any effect of parasitaemia on fuel load.
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    First record of dorsal and anal fin deformities in silver pomfrets, Pampus argenteus (Stromateidae, Actinopterygii)
    (Murcia : Universidad de Murcia, Servicio de Publicaciones, 2010) Al-Mamry, Juma M.; Jawad, Llaith A.; Al-Rasady, Issam H.; Al-Habsi, Sahoud H.
    The information on anal fin deformity is scanty and there is a paucity of knowledge regarding the causes behind such anomaly. Although, some information on the pterygiophore deformity in fish is available, nothing is known about the complete absence of those structures. In the present study, a description of the case of absence of the pterygiophores supporting the anal fin in silver pomfrets, Pampus argenteus (Euphrasen, 1788) collected from the Omani coasts of the Arabian Gulf is given.