Histology and histopathology Vol. 6, nº 1 (1991)
Ir a Estadísticas
Permanent URI for this collection
Browse
Recent Submissions
- PublicationOpen AccessPresence and possible function of Zn in the hyaline cartilage(Murcia : F. Hernández, 1991) Pérez Castejón, M.C.; Barral, M.J.; Vera Gil, A.; Pérez Castejón, M.J.; Lahoz, M.Using histochemical and autoradiographical techniques the location of Zn in the hyaline cartilage of the Wistar rat was studied. A triple location was observed in the territorial matrix; in the chondrocyte; and in the perichondrio (cellular area). We believe that the molecules which were observed carrying Zn could be alkaline phosphatase, timidin-kinase and chondroitinsulphuric acid.
- PublicationOpen AccessUltrastructure of the rat hippocampus after isobaric respirative hyperoxia(Murcia : F. Hernández, 1991) Manthos, A.; Tsolaki, M.; Kaidoglou, K.; Alvanou, A.; Hatjinikolaou, K.; Hatjiagorakis, A.; Giala, M.; Foroglou, Ch.After exposing rats to an environment of isobaric h,v ~eroxia.th e ~iltrastructurala lterations of the L hippocampus were studied. No major alterations were found in the nerve cells. Of importante was the moderate osmiophilia and the spindle-like transformation of the mitochondria. Vacuolated synapses and neuraxons were found, containing amorphous material. Astrocytic perivascular end feet were found vacuolated in many places. Many endothelial cells of the capillaries presented high osmiophilia, which sonletimes prevented structural details. Quantitatively, the findings were proportionally related to the time of exposure in the pure oxygen atmosphere (24,48 and 65 hours)
- PublicationOpen AccessUltrastructural changes induced by a-sarcin in a human pulmonary tumor grown in naked mice(Murcia : F. Hernández, 1991) Mohamed-Ali, H.; Kolkenbrock, H.J.; Hecker-Kia, A.; Hinz, N.; Ulbrich, N.a-Sarcin is a cytotoxic polypeptide produced by Aspergillus giganteus. It suppresses protein synthesis in yeast and wheat germ extracts and has a purine-specific RNase activity. The substance has been tested for its antitumor properties in a series of induced tumor systems in mice such as sarcoma and carcinoma among others. Although some of the in vitro effects of a-Sarcin on certain cellular components have been elucidated, the biological effects leading to cellular damage are still obscure. In this work we analysed the morphological changes in tumor cells derived from human pulmonary adenocarcinoma heterotransplanted and grown in naked mice, induced shortly (24 hours) after a single intratumoral injection of U-Sarcin (0.4 mgltumor). The results obtained were: 1) swelling of mitochondria; 2) cell necrosis with partial removal of necrotic cells by phagocytosis; 3) thickening of interlobular connective tissue; 4) hyperplasia of globet-cell-like clear cells. The mode of action concerning these cellular changes is presently uncertain. In view of the severity of these structural alterations it seems conceivable that a-Sarcin may enter the cell undergoing interactions with different intracellular structures. This would require a selective membrane permeabilization, perhaps induced upon formation of complexes with negatively-charged membrane phospholipids
- PublicationOpen AccessThe alveolar pores of Kohn in young postnatal rat lungs and their relation with type II pneumocytes(Murcia : F. Hernández, 1991) Van Meir, FransIn order to obtain more information on the development, morphology and function of the pores of Kohn, the lungs of Wistar rats are studied during their early postnatal period, up to 3 weeks of age, by scanning and transmission electron microscopy. The substantial development of the interalveolar pores on days 14 and 21 coincides with the period of septal rearrangement when secondary interalveolar septa become lengthened and thinner. The high frequency of transseptal type 11 pneumocytes from day 7 onwards, and their typical localization near the pores of Kohn at this period of lung development especially suggests that type 11 pneumocytes are engaged in the formation of the pores of Kohn. During early lung development, the pores of Kohn seem to serve as passageways for alveolar macrophages.
- PublicationOpen AccessAge-related quantitative changes in the organelles of rat neocerebellar Purkinje cells(Murcia : F. Hernández, 1991) Monteiro, R.A.F.