Histology and histopathology Vol. 6, nº 1 (1991)
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- PublicationOpen AccessPresence and possible function of Zn in the hyaline cartilage(Murcia : F. Hernández, 1991) Pérez Castejón, M.C.; Barral, M.J.; Vera Gil, A.; Pérez Castejón, M.J.; Lahoz, M.Using histochemical and autoradiographical techniques the location of Zn in the hyaline cartilage of the Wistar rat was studied. A triple location was observed in the territorial matrix; in the chondrocyte; and in the perichondrio (cellular area). We believe that the molecules which were observed carrying Zn could be alkaline phosphatase, timidin-kinase and chondroitinsulphuric acid.
- PublicationOpen AccessExperimental thioacetamide-induced cirrhosis of the liver(Murcia : F. Hernández, 1991) Muñoz Torres, E.; Paz-Bouza, J.I.; López Bravo, A.; Abad Hernández, M.M.; Carrascal Marino, E.Hepatic cirrhosis is a complex disease in which several biological, biochemical and chemical alterations are combined, none of these alone being sufficient for diagnosis. The morphological characteristics of the final stages of cirrhosis are well known, but the initial lesions and intermediate stages still have not been fully clarified. An experimental model of hepatic cirrhosis by chronic administration over 30 weeks of thioacetamide (50 mglkg twice weekly) to female Wistar rats has been produced. In a macroscopic, microscopic and ultrastructural study. The different lesions that appeared were evaluated according to the dose of the toxic agent administered up, until hepatic cirrhosis was finally installed; this was after 60 doses of the toxic agent (30 weeks). Discussion is made of the different types of administration and the doses employed to obtain a suitable survival rate for these cases; in our experiments this was 95%. It has been demonstrated in both human and experimental pathology that once the disease itself has been installed, currently there is no rational or useful treatment for it. A beneficial effect has been demonstrated for certain substances, improving the initial and intermediate lesions. so we conclude by stating that it is necessary to further study the hepatic lesions preceeding cirrhosis. Knowledge of these lesions could form the basis for establishing a useful and rational therapy for such cases.
- PublicationOpen AccessMorphological abnormalities in the sural nerve from patients with peripheral vascular disease(Murcia : F. Hernández, 1991) Rodríguez-Sánchez, C.; Medina sánchez, M.; Malik, Rayaz A.; Ah-See, A.K.; Sharma, A.K.The present paper has been written in order to determine the morphological alterations in the sural nerve from patients with chronic arteriosclerotic occlusive disease. Eight patients with Peripheral Vascular Disease (PVD) and six age-matched control subjects were studied. Morphometric data revealed two groups of patients, one of them with mild disease (n=5), and the other one with severe damage (n=3,) consisting in loss of myelinated fibres and increase in the number of small fibres (p < 0.005). Teased nerve fibres and electron microscopic studies also showed two types of patients, with respect to the myelin or the axonal alterations. The unmyelinated fibre population was affected equally in both groups. In conclusion, this study supports the idea that ischemia is able to cause structural alterations in the peripheral nerve, and that it can play a role in the development of neuropathy.
- PublicationOpen AccessLectins as differentiation markers of human gliomas(Murcia : F. Hernández, 1991) Figols, J.; Cervós-Navarro, J.; Madrid Cuevas, Juan FranciscoThe lectins Concanavalin A (Con A). Ricinus communis agglutinin (RCA-1), Peanut agglutinin (PNA) and Wheat germ agglutinin (WGA) as well as the irnmunomarkers for glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) were used in a series of 21 glial turnors (4 pylocytic astrocytornas, 5 grade 11 astrocytornas, 3 anaplastic astrocytornas, 4 glioblastornas and 5 oligodendrogliornas). ConA binds to al1 tumoral astrocytes in low grade astrocytomas, as well as to well differentiated tumoral astrocytes in anaplastic astrocytomas and glioblastornas. RCA-1 has a similar behaviour. PNA, and to a lesser degree WGA, binds selectively to the oligodendroglial plasma membrane in well differentiated oligodendrogliomas. The results suggest that these lectins are markers of differentiation in gliomas rather than of malignancy.
- PublicationOpen AccessUltrastructure of the rat hippocampus after isobaric respirative hyperoxia(Murcia : F. Hernández, 1991) Manthos, A.; Tsolaki, M.; Kaidoglou, K.; Alvanou, A.; Hatjinikolaou, K.; Hatjiagorakis, A.; Giala, M.; Foroglou, Ch.After exposing rats to an environment of isobaric h,v ~eroxia.th e ~iltrastructurala lterations of the L hippocampus were studied. No major alterations were found in the nerve cells. Of importante was the moderate osmiophilia and the spindle-like transformation of the mitochondria. Vacuolated synapses and neuraxons were found, containing amorphous material. Astrocytic perivascular end feet were found vacuolated in many places. Many endothelial cells of the capillaries presented high osmiophilia, which sonletimes prevented structural details. Quantitatively, the findings were proportionally related to the time of exposure in the pure oxygen atmosphere (24,48 and 65 hours)