Anales de biología Vol. 5 (1985) Biología general, 1
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- PublicationOpen AccessDeterminación fluorimétrica de ácido indolil-3-acético en altramuz(Universidad de Murcia, Servicio de Publicaciones, 1985) Sánchez Bravo, José; Ortuño Tomás, Ana María; Acosta Echevarría, Manuel; Sabater, F.; Facultad de BiologíaThe quantification of the phytohormone, indolil-3-acetic acid l]AAJ. in me.thanolic extracts of etiolated hypocotyls of Lupíne by a fluorimetric method, has been investigated. The applícation of the established criteria for the use of thís method shows that the observed fluorescence corresponds to the fluorescent lAA derivative. and that the other components of the methanolic extracts do not interfere in the measure. So, it is possible to quantify directly the !AA in the methanolic extracts without previous purification.
- PublicationOpen AccessMolecular virology.:Reseña blbliográfica(Universidad de Murcia, Servicio de Publicaciones, 1985) Gacto Fernández, Mariano José; Vicente Soler, Jerónima; Facultad de Biología
- PublicationOpen AccessParámetros hematológicos de Rana perezi (Amphibia: Salientia)(Universidad de Murcia, Servicio de Publicaciones, 1985) Martínez López, Francisco Javier; Mendiola López, Pilar; Costa Ruiz, Jorge de; Facultad de BiologíaAs part of a wider research on respiratory properties of Rana perezi blood, the hematological parameters of this species have been measured. The study of blood O ,-transporting proteins (hemoglobin, Hb) and plasmatic proteins has also been initiated. Using fifteen females weighting 235 g (15.3-31.8), !he following results have been obtaíned: hematocrit (He(): 25. 7 :+:3.2 (ne, 15): Red blood cell count (RBC): 482:+: f 54x 1()3/mm1 (n=I4); Hb concentration: 5. 19:+:0.99 g/100 mi (n=9); mean corpuscYlar volume (MCV): 533.29 μ3; mean corpuscular Hb concentration (MCHC): 20.15% and mean corpuscular Hb (MCH): 107.5 pg. The electrophoresis of the Hb in 5% polyacrylamide gels showed two bands. Their quantification gave 72.38:+: ±20.19% (n=ll) for the anodic band and 27.62:t20.19 (n=ll) for the catodic one. Electrophoresis of plasma was performed in different kinds of polyacrylamide gels. the best obtained being in 7.5 and 8.5% gel. Electrophoretic behavíour of plasma proteins seems to be similar to that described in terrestrial vertebrates. These results are discussed and compared with data from other species belonging to Bufo. Hy/a and Rana genera.
- PublicationOpen AccessMetabolismo de AIA-14C por secciones de plántulas de altramuz: influencia de diversos factores(Universidad de Murcia, Servicio de Publicaciones, 1985) Sánchez Bravo, José; Montalbán, A.; Acosta Echevarría, Manuel; Sabater, F.; Facultad de BiologíaThe auxinic metabolism in etiolated hypocotyls of Lupine is studied by incubation of sections in solutions of lAA-14C,. The prnduct:s are sequentially extracted with 95•/r ethanoL water and I M NaOH. Thé radiactivity in each fraction depends on different factors such as: time of incubation. hormone concentration. order of use of the solvents. permanence of auxin in the tissue and localization of the section in the hypocotyls. This suggests that conjugation or degradatíon of auxin can be affected and so the suspected equilibrium between the ditferent forms of auxins is altered.
- PublicationOpen AccessRespiración endócena de Ceriporla bresadolae (bdot. et galz.) bond. & sing. (Aphyllophorales: Basidiomycotina)(Universidad de Murcia, Servicio de Publicaciones, 1985) Martínez González, M. C.; Honrubia García, Mario; Facultad de BiologíaMeasures of Q02 have been made with Ceriporia bresadolae at different stages of growth and different temperatures. Values of respiratíon have been measured with suspensions of mycelium from C. bresadolae to which dífferent substrates such as piruvate, glucose, acetate and maltose were added exogenously. Strong activity has been obtained with piruvate and maltose. The respiratory quotient value was found to be 0.72.