Histology and histopathology Vol. 2, nº 4 (1987)
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- PublicationOpen AccessInterstitial collagen in alcoholic human liver(Murcia : F. Hernández, 1987) Stenbäck, F.; Wasenius, Veli-Matti; Sotaniemi, Eero A.The occurrence and intensity of staining for specific antibodies against the aminoterminal propeptide of type 111 procollagen (PIIIP), which is indicative of the synthesis and the degradation of that collagen type, was studied in sections from normal and alcoholic livers and compared with serum PIIIP levels, serum antipyrine clearance, fibronectin distribution and morphology as revealed by conventional stains and electronmicroscopy. Positive staining for PIIIP and fibronectin was observed in the perisinusoidal space of the normal liver and in portal tracts. In alcohol-induced fatty liver positive staining increased around the central veins, in alcoholic hepatitis increased staining reaction was seen to a limited extent in areas of cell injury. Extensive reticulin and PIIIP-positive areas were found in the periportal interstitium of the cirrhotic livers and in large fibrotic areas extending into the surrounding parenchyma in cases of active disease. The results show a distinct relationship between collagen type 111 metabolism, morphologically detectable hepatic injury and liver cell function tests, with tissue deposition occurring later in the disease process than biochemically detectable serum collagen levels and signs of altered liver cell function.
- PublicationOpen AccessMorphometric study of hepatic ultrastructure in alcoholic hepatitis(Murcia : F. Hernández, 1987) Chen, T.S.; Murphy, D.P.; Marquet, G.; Chedid, A.; Mendenhall, C.L.; Rabin, L.; Veterans Administration Cooperative Study Group on Alcoholic HepatitisWe undertook a morphometric analysis of hepatocellular organelles in an attempt to correlate their changes with the clinical stages of patients.with alcoholic hepatitis. Although hepatic ultrastructural alterations did not correlate with disease severity, we found significant differences between patient and control groups in the measured parameters of non-organelle cytoplasm, mitochondria, SER, RER, glycogen, and lipid.
- PublicationOpen AccessUltrastructure of the tubular nephron of Testudo graeca (Chelonia). A comparison between hibernating and non-hibernating animals(Murcia : F. Hernández, 1987) Zuasti, A.; Ferrer, C.; Ballesta Germán, José; Pastor García, Luis MiguelThe tubular nephron of hibernating and nonhibernating specimens of Testudo graeca (Chelonia) was studied by means of conventional light and electron microscopy and histochemistry. The tubular nephron was composed of proximal, intermediate, distal and collecting tubules in both hibernating and nonhibernating animals. The cells of the proximal tubule showed long microvilli, cytoplasmic vacuoles, a developed endoplasmic reticulum and abundant mitochondria. Fat droplets were also observed. The intermediate segment was lined by ciliated and nonciliated cells. The lining cells of the distal tubule presented few microvilli, abundant dense mitochondria and clear vesicles of mucous appearance in the terminal portion. Collecting ducts are composed of mucous and non-mucous cells. Mucous cells presented strong reaction to the histochemical techniques detecting sialoand sulpho-mucins. During hibernation, a progressive vacuolar degeneration of the endoplasmic reticulum was observed in al1 the segments of tubular nephron, which may be caused by a massive intake of extracellular water into the cell.
- PublicationOpen AccessUltrastructural relationship of quadriceps muscle degeneration with a distant peroneal nerve conduction in human myotonia dystrophica(Murcia : F. Hernández, 1987) Sinha, Akhouri A.; Olson, Norman D.; Nuttall, Frank Q.The association of motor nerve conduction velocity (MNCV) to (1) duration of symptoms, (2) deep tendon reflex responses, (3) clinical muscle atrophy, and (4) ultrastructure of quadriceps muscle was studied in 18 patients with myotonia dystrophica of Steinert and nine normal controls. These patients had neither diabetes mellitus nor any other type of muscle dystrophy. Ultrastructural features of muscle fibers and intercellular spaces between atrophic fibers provided a basis for identifying degenerative changes and evaluating them semi-quantitatively. Our study indicates presence of an association between the pattern of muscle degeneration and both MNCV (correlation coefficients, y=+0.60) and duration of symptoms (y=-0.62), but not between MNCV and duration of symptoms (y=+0.28). Further analysis of the association between the degeneration of quadriceps and the MNCV of a distant peroneal nerve (which does not innenate quadriceps) suggested that the systemic nerve degeneration occurred in some groups of myotonia patients. Our study indicates that while in some patients the muscle degeneration may have been associated with the impairment of neurogenic elements, in others it occured in the absence of any MNCV abnormality. Our findings favor the role of both neuropathic and myopathic factors in the muscle degeneration seen in myotonia dystrophica.
- PublicationOpen AccessExpression of cytokeratin and neuron-specific enolase in small cell carcinomas of the lung(Murcia : F. Hernández, 1987) Kitinya, James N.; Mitsuru Kingo; Kenzo TanakaUsing a polyclonal antibody against human epidermal keratins and a monoclonal antibody against cytokeratins characteristic of simple epithelia, and the Avidin-Biotin system of immunohistochemistry, we have demonstrated cytokeratin expression in 46% and in 60% of small cell carcinomas of the lung at autopsy respectively. The latter gave a diffuse stronger reaction product than the polyclonal antibody. The results suggest that there is a cytokeratin rich and a cytokeratin poor type of small cell carcinoma. Neuron-specific enolase immunohistochemistry was positive in 60% of the cases. Coexpression with cytokeratin was seen in ten cases (30%). The expression of cytokeratin and neuronspecific enolase in small cell carcinomas strongly suggests that they are of an epithelial origin, but are capable of neuroendocrine differentiation.
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