Histology and histopathology Vol. 4, nº 1 (1989)

Ir a Estadísticas

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http://hdl.handle.net/10201/177573

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Ultrastructural localization of Cu, Zn-SOD in hepatocytes of patients with various liver diseases
(Murcia : F. Hernández, 1989) Saito, Takafumi; Shinzawa, Haruhide; Togashi, Hitoshi; Wakabayashi, Hiroto; Ukai, Katsuaki; Takahashi, Tsuneo; Ishikawa, Makoto; Dobashi, Michio; Imai, Yutaka
The ultrastructural localization of copper, zinc-superoxide dismutase (Cu, Zn-SOD) in the liver of patients with acute hepatitis, chronic hepatitis, liver cirrhosis and alcoholic fatty liver was studied by means of the indirect immunoperoxidase technique. In hepatocytes Cu, Zn-SOD was found to be localized in perinuclear cisternae, rough endoplasmic reticulum (rER), vesicles and Golgi apparatus. The Cu, Zn-SOD was also detected around the lipid droplets in hepatocytes as well as on the cytoplasmic membrane in cases of liver cirrhosis. These findings suggest that Cu, Zn-SOD is produced in the rER in hepatocytes and protects the cells from cellular injury caused by superoxide anion radical in various disorders of the liver.
Open Access
Rabbit pasteurellosis: Respiratory and renal pathology of control and immunized rabbits after challenge with Pasteurella multocida
(1989) Al-Lebban, Z.S.; Kruckenberg, S.; Coles, E. H.
Gross and microscopic lesions of pasteurellosis were studied in control and immunized pasteurella-free rabbits after challenge with virulent Pasteurella multocida. Pathologic responses were compared in rabbits immunized intravenously or mucosally with P. multocidaor with J5, a cross protective core LPS mutant of E. coli Al1 rabbits were challenged conjunctivally with approximately 2xLD,, of P. multocida. Rabbits were necropsied and examined for histopathology of the respiratory tract and kidneys. Lung lesions varied in severity depending on the duration of the disease, the route of vaccination, and the vaccine used. The most severe lung lesions occurred in rabbits vaccinated intravenously with P. multocida and challenged with the same strain. Some of these rabbits had purulent bronchopneumonia and pleuropneumonia. Lung lesions were absent or less severe in rabbits vaccinated by a mucosal (aerosol, conjunctival) roiite and in unvaccinated controls. In these animals there was no bronchopneumonia or pleuropneumonia, and bronchiolitis, if present, was less severe. Kidney lesions were found only in rabbits vaccinated intravenously. There was an interstitial nephritis. some collagen deposition. mononuclear cell infiltration, and a loss of tubular architecture in the cortex. Some glomeruli were affected. These results indicate that intravenous immunization contributes to the formation of lesions whereas mucosal immunization prevented lesion formation to some degree.
Open Access
The effects of cyclophosphamide on the prolactin cells of the normal rat
(Murcia : F. Hernández, 1989) Poblador, María S.; Rojas, Carmen; Raya, Antonio; Quiralte, Joaquín; Casares, José A.; Lancho Alonso, José Luis
Cyclophosphamide administered at doses of 400 mg/m2/5 days with sacrifice two days later, and 200 mg/m2/5 days with a 21-day break and a further five days of treatment, with sacrifice two days later, provokes similar effects. Functional activity is less marked in untreated and control animals than in treated ones, as the hormone data shows. But the ultrastructure of PRL cells in treated animals indicates the existence of clear hormone synthesis activity, evident in the fusion and clustering of granules at differing stages of maturity, etc.
Open Access
Microscopic correlates of adaptive cytoprotection in an ethanol injury model
(Murcia : F. Hernández, 1989) Schmidt, Carmen L.; Smith, Gregory S.; Miller, Thomas A.
The present study histologically investigated the efficacy of pretreating rat gastric mucosa with the mild irritants, 10% and 25% ethanol (EtOH), against the known damaging effects of 100% EtOH. Fasted rats received 1 m1 of either water, 10% EtOH, or 25% EtOH by orogastric intubation. Fifteen minutes later, a portion of these animals was sacrificed and tissue samples of the oxyntic region of the stomach were excised and processed for quantitative histologic analysis. Remaining animals received a 1 m1 oral bolus of the necrotizing agent, 100% EtOH. Five minutes later, these animals were sacrificed and tissues were prepared in a like manner. In a separate series of experiments, the aforementioned protocols were repeated, except that al1 animals received the prostaglandin synthetase inhibitor, indomethacin (5.0 mg/kg intraperitoneally), 30 min before administration of the mild irritant. Microscopically, the administration of water or 10% EtOH alone caused a small and comparable amount of superficial injury to the gastric mucosa. Moreover, both substances failed to induce protection in stomachs subseqently exposed to 100% EtOH. Indomethacin pretreatment did not significantly alter any of these findings. In marked contrast, 25% EtOH alone elicited a substantial degree of superficial damage to the gastric mucosa. Nevertheless it significantly reduced the depth of injury in animals subsequently challenged by 100% EtOH. Indomethacin failed to aggravate the effects of 25% EtOH alone, but partially inhibited the protective effect of this mild irritant against 100% EtOH induced damage. Our findings indicate that adaptive cytoprotection is a real phenomenon that can be demonstrated microscopically. Such protection is limited primarily to the deep mucosa1 layers (i.e. gastric glands), appears in part to be prostaglandin mediated and seems to require the generation of moderate surface cell damage (as occurred with 25% EtOH, but not 10% EtOH) to induce its initiation.
Open Access
Study of h-TSH immunoreactive adenohypophyseal cells following treatment with methymazole
(Murcia : F. Hernández, 2011-01-18) Carretero, J.; Sánchez, F.; Torres, J. L.; Blanco, E.; Riesco, J. M.; Vázquez, R.
The TSH-producing adenohypohyseal cells of Wistar rats were studied after treatment with orally administered methymazole by the PAP immunocytochemica1 method. These cells were compared with those of normal animals. The effects of methymazole were different in the females to those observed in males. In both sexes. the numerical density (number of TSH cells per 1000 pm2) increased after treatment. The cellular and cytoplasmic areas increased in the females while they decreased in the males. The nuclear area and the nuclear area/cytoplasmic area ratio increased in the males and decreased in the females following treatment with methymazole. This treatment caused the appearance of large. intensely stained cells, with an eccentric nucleus and cytoplasmic processes accompanied by weakly stained cells that were situated close to blood vessels.

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