Browsing by Subject "Sequencing"

Now showing 1 - 3 of 3
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    Expression of IZUMO1 and JUNO in the gonads of domestic cats (Felis catus)
    (Elsevier, 2024-05) Sanguansook, P; Rodprasert, W; Sawangmake, C; Gimeno, L; Ferrán, JL; Soria-Monzó, P; Avilés, M; Izquierdo Rico, MªJosé; Chatdarong, K; Biología Celular e Histología
    Because of the time-consuming nature of surgical neutering and the rapid rate of reproduction among domestic cats, it is crucial to investigate alternative, nonsurgical methods of contraception for this species. Sperm protein IZUMO1 and its oocyte receptor JUNO have been proposed as potential targets for nonsurgical contraceptives. This study aimed to demonstrate (1) the protein coding sequence of feline IZUMO1 and JUNO, (2) gene expression in specific organs by measuring mRNA levels in different visceral tissues, and (3) the expression of IZUMO1 and JUNO during sperm maturation and folliculogenesis, respectively. Amplification for sequencing of feline IZUMO1 and JUNO was performed using the RT-PCR method. Levels of gene expression in different tissues were evaluated using real-time PCR. In situ hybridization was performed to localize JUNO mRNA in ovarian tissues. The complete coding sequences of IZUMO1 and JUNO were obtained and analyzed. A comparison between protein orthologs demonstrated the conservation of IZUMO1 and JUNO in Felidae. The real-time PCR results from various visceral organs indicated that IZUMO1 was significantly higher in the testis than in other organs, whereas JUNO was significantly higher in the ovary than in other organs. Expression of IZUMO1 was found to be higher in the testes than in the caput, corpus, and cauda of epididymides. In situ hybridization revealed that JUNO mRNA was in the ooplasm and nucleus of the primordial, primary, secondary, and antral follicles. Importantly, this was the first study to demonstrate the IZUMO1 and JUNO genes in the testis and ovary of cats. The results are useful for future research related to these genes and for developing contraceptives against these targets.
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    Implementación del e-learning en la enseñanza del modelo “Proceso de Atención de Enfermería” para la formación universitaria
    (Universidad de Murcia, Servicio de Publicaciones, 2014) García Puig, María Elena
    Se ha detectado la necesidad de implementar el e-learning en la formación universitaria de enfermería. Para ello, esta investigación aplica el diseño instruccional al proceso de aprendizaje de un modelo profesional del cuidado, cuyo centro son las competencias a desarrollar por el estudiante. A partir de éstas se ha secuenciado el conocimiento a través de la Teoría de la Elaboración y la Técnica de Análisis de los Contenidos. Así mismo, en base a estos elementos se ha diseñado una serie de tareas teniendo en consideración la Técnica de Análisis de las Tareas y el Método de Simplificación de las Condiciones (SCM). Por otro lado, se ha diseñado y creado un objeto de aprendizaje a través del patrón EASA del software GLO Maker. Además en el planteamiento y diseño de la instrucción se ha tenido en consideración los cinco principios de David Merril. Todo ello orientado a un aprendizaje constructivista y una modalidad de enseñanza instruccional virtual
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    Liquid biopsy of circulating tumor cells: From isolation, enrichment, and genome sequencing to clinical applications
    (Universidad de Murcia, Departamento de Histología e Histopatología, 2025) Tan Keqin; Zhu Hong; Ma Xuelei; Biología Celular e Histología
    Circulating tumor cells (CTCs), shed from primary tumors into the bloodstream, play a crucial role in metastasis and hold great potential in cancer diagnosis, prognosis, and treatment monitoring. Conventional CTC detection using epithelial biomarkers like epithelial cell adhesion molecule (EpCAM) for immunocapture overlooks mesenchymal-like CTCs with high metastatic potential, spurring the development of non-immunocapture technologies that use biophysical traits for enrichment. Innovations in microfluidic platforms and multi-parametric sorting improve isolation efficiency and address related challenges. Breakthroughs in single-cell genomic and transcriptomic sequencing enable in-depth molecular characterization of CTCs. Clinically, CTC enumeration and molecular profiling are emerging as real-time tools for assessing therapeutic response and predicting outcomes, especially in metastatic breast, prostate, and colorectal cancers. This review focuses on CTC isolation, enrichment techniques, their applications in different tumors, downstream analysis progress, and potential in precision medicine