Browsing by Subject "Rejection"

Now showing 1 - 4 of 4
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    MicroRNA expression changes in kidney transplant: diagnostic efficacy of miR-150-5p as potential rejection biomarker, pilot study
    (MDPI, 2021-06-22) Alfaro, Rafael; Legaz Pérez, Isabel; Jiménez Coll, Víctor; El kaaoui El band, Jaouad; Martínez Banaclocha, Helios; Galián, José Antonio; Parrado, Antonio; Mrowiec, Anna; Botella, Carmen; Moya Quiles, María Rosa; Boix, Francisco; Peña Moral, Jesús de la; Minguela, Alfredo; Llorente, Santiago; Muro, Manuel; Ciencias Sociosanitarias
    Background: The kidney allograft biopsy is considered the gold standard for rejection diagnosis but is invasive and could be indeterminate. Several publications point to the role of miRNA expression in suggesting its involvement in the acceptance or rejection of organ transplantation. This study aimed to analyze microRNAs involved in the differentiation and activation of B and T lymphocytes from kidney transplant (KT) patients’ peripheral blood leukocytes to be used as biomarkers of acute renal rejection (AR). Methods: A total of 15 KT patients with and without acute rejection (AR/NAR) were analyzed and quantified by miRNA PCR array. A total of 84 miRNAs related to lymphocyte differentiation and activation B and T were studied. The functions and biological pathways were analyzed to predict the potential targets of differential expressed miRNAs. Results: Six miRNA were increased in the AR group (miR-191-5p, miR-223-3p, miR-346, miR-423-5p, miR-574-3p, and miR-181d) and miR-150-5p was increased in the NAR group. In silico studies showed a total of 2603 target genes for the increased miRNAs in AR, while for the decrease miRNA, a total of 1107 target-potential genes were found. Conclusions: Our results show that KT with AR shows a decrease in miR-150-5p expression compared to NAR, suggesting that the decrease in miR-150-5p could be related to an increased MBD6 whose deregulation could have clinical consequences.
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    Solid phase-based cross-matching for solid organ transplantation: Currently out-of-stock but urgently required for improved allograft outcome
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Schlaf, Gerald; Bau, Daniela; Horstmann, Nathalie; Sawers, Gary; Altermann, Wolfgang
    Transplant recipients who have undergone sensitizing events, such as pregnancy, blood transfusion or previous transplants, frequently develop antibodies directed against the highly polymorphous human leukocyte antigen (HLA)-molecules. These pre-formed, donor-specific antibodies (DSA) present a high risk of causing organ failure or even complete loss of the grafted organ as a consequence of antibody-mediated, hyper-acute or acute allograft rejection. In order to detect DSA, the so-called functional complement- dependent lymphocytotoxicity assay (CDC-XM) was established about 50 years ago. Although effective in improving the outcome of solid organ allo-grafting, for the last ten years this assay has been controversially discussed due to its low sensitivity and especially because of its high susceptibility to various artificial factors, which generally do not yield reliable results. As a consequence, novel immunochemical test systems have been developed using ELISA- or bead-based solid phase assays as replacements for the traditional CDC- based assays. Because these assays are independent of single or vital cells, which are frequently not available, they have provided an additional and alternative diagnostic approach compared with the traditional CDC-based and flow-cytometric analyses. Unfortunately, however, the AMS-ELISA (Antibody Monitoring System), which was the first system to become commercially available, was recently discontinued by the manufacturer after seven years of successful use. Alternative procedures, such as the AbCross-ELISA, had to be either considerably modified, or did not yield reliable results, as in the case of the Luminex-based assay termed DSA. We draw the conclusion that due to the unique features and fields of application reviewed here, the implementation of solid phase cross-matching still represents an urgent requirement for any HLA-laboratory’s routine tasks
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    Soporte social en bullying. Análisis de la soledad de la víctima
    (2014-01-01) Cerezo Ramírez, Fuensanta
    Studies about bullying indicate that this is an interpersonal relationships problem and that the victim' isolation is a major risk factor. The main objective is to analyze the relation between being bullying victim and social ascendance. The Bull-S test was completed by 1478 of Secondary Schools (47.8% males,  48.4% females) from 11-18 years old (X= 14.48; dt= 1.43) from the Region of Murcia (Spain). 19.4% was implied in bullying: 129 victims, 145 bullies and 13 aggressive-victims. Sociometric analysis confirmed that those pupils that are implied are worse valuated that no implied and being victimized is related to rejection, expectative of being rejected and exclusion. In general, bullying victims are not more isolated, but they are the worst perceived. These results alert from the necessity of improve detection strategies and include interpersonal relationships evaluation as risk or preventive bullying factor.
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    The resistant effect of slurs: A nonpropositional, presuppositional account
    (Universidad de Murcia. Servicio de Publicaciones, ) Moreno Zurita, Alba; Pérez Navarro, Eduardo
    The aim of this paper is to account for the resistance to cancelation, rejection, and retraction exhibited by slurs. The kind of explanation we offer is a presuppositional one. Like the most recent presuppositional accounts, moreover, ours is a nonpropositional presuppositional proposal. Our view is that, to be felicitous, utterances of sentences featuring slurs require certain components to be part of the common ground, but these components are not propositions, but worldorderings.