Browsing by Subject "Oestrus ovis"
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- PublicationOpen AccessDevelopment and characterization of 15 novel polymorphic microsatellite loci for two important bot flies (Diptera, Oestridae) by next-generation sequencing(Springer, 2020) Aleix-Mata, Gaël; Expósito, Miriam; Ruiz Ruano, Francisco J.; López Beceiro, Ana María; Fidalgo, Luis E; Martínez Carrasco-Pleite, Carlos; Ruiz de Ybáñez Carnero, María del Rocío; Boos, Mathieu; Pérez, Jesús M.; Sánchez, Antonio; Sanidad AnimalCephenemyia stimulator and Oestrus ovis are two important parasitic bot flies (Oestridae) species causing myiasis, with a potential negative impact on the welfare of the host. Using next-generation sequencing approach and bioinformatics tools, a large panel of possible microsatellites loci was obtained in both species. Primer pairs were designed for 15 selected microsatellite loci in C. stimulator and other 15 loci in O. ovis for PCR amplification. Loci amplification and analysis were performed in four populations of each species. The results demonstrated that all selected loci were polymorphic, with the number of alleles ranging from 2 to 6 per locus in C. stimulator and 3 to 13 per locus in O. ovis. This is the first time to describe these microsatellite loci for C. stimulator and O. ovis. These two sets of microsatellite markers could be further used for biogeographic and population genetics studies.
- PublicationOpen AccessFirst report of oestrosis in aoudad from southeastern Spain(2017-05-15) Barroso, Patricia; Ruiz de Ybáñez Carnero, María del Rocío; Martínez Carrasco-Pleite, Carlos; Gens, M.J.; Escribano, F.; Sánchez, A.; Pérez, J.M.; Sanidad AnimalIn spring 2016, we analyzed the skull of 44 aoudads (Ammotragus lervia) from Sierra Espuña Regional Park (SE Spain) for the presence of oestrid larvae. Oestrus ovis larvae were found in 27.3% of sampled hosts, with a mean intensity of 1.5 ± 6.1 larvae/per parasitized host (range 1–21). To our knowledge, this is the first report of oestrosis affecting this host species.
- PublicationOpen AccessPresencia de Oestrus ovis (Linnaeus 1761) en el ganado ovino de la región de Murcia (sureste de España)(Sociedad Española de Parasitología (SEP), 2004-11-18) Garijo Toledo, M. M; Alonso de Vega, Francisco; Martínez Carrasco-Pleite, Carlos; Ruiz de Ybáñez Carnero, María del Rocío; Sanidad AnimalEn el presente trabajo se ha evaluado la parasitación por estadios larvarios de Oestrus ovis (O. ovis) en el ganado ovino de la Región de Murcia (Sureste español). Se inspeccionaron un total de 181 cabezas procedentes de ovejas adultas recogidas en un matadero regional. De ellas, 69 (38,1%) estaban parasitadas por el díptero. Se contabilizaron 1.807 larvas, de las cuales, el 98,8%estaban vivas. La carga media por animal fue de 26,2 larvas. Se observó un predominio de larvas de primer estadio sobre las de segundo y tercer estadio. El 87,5% de las larvas se localizaron en la concha nasal ventral y en el meato medio. Las dos comarcas ganaderas con mayor prevalencia de O. ovis fueron el Altiplano y el Valle del Guadalentín. El menor número de animales parasitados se encontró en las comarcas de la Vega del Segura y el Campo de Cartagena.
- PublicationOpen AccessPrevalence of Oestrus ovis in small ruminants from the eastern Iberian Peninsula. A long-term study(Wiley, 2023-01-24) Garijo Toledo, María Magdalena; Sansano-Maestre, José; Ahuir-Baraja, Ana Elena; Martínez Carrasco-Pleite, Carlos; Alonso de Vega, Francisco Domingo; Llobat, Lola; Ruiz de Ybáñez Carnero, María del Rocío; Sanidad AnimalOestrus ovis is an obligate parasite that causes myiasis in domestic ruminants, being commonly found in the Mediterranean area. From 2009 to 2019 a total of 3476 heads of culling sheep and goats from the Mediterranean coast of Spain were examined for the presence of O. ovis. The total prevalence was 56.3%, significantly higher in sheep than in goats (61.2% and 43%, respectively). Differences were found in the mean annual prevalence, with the highest value being registered in 2018 (61.7%) and the lowest in 2012 (50.3%). Autumn, for sheep, and winter, for goats, were the seasons with the highest number of infested specimens. Temperature, but not rainfall, was found to be associated with prevalence (p < 0.05). Most L1 were found in the anatomic region I (septum, meatus, and ventral conchae), while L2 and L3 were mainly located in regions II (nasopharynx, ethmoid labyrinth, and dorsal conchae), and III (sinuses). The overall intensity was 12.8 larvae per head, significantly higher in sheep (13.3) than in goats (3.5). Our results confirm the high prevalence of O. ovis in sheep and goats in this geographic area over the last decade, with the trend increasing in recent years in association with higher mean temperatures