Browsing by Subject "Matrix"

Now showing 1 - 5 of 5
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    Carbamazepine overdose after psychiatric conditions: A case study for Postmortem Analysis in Human Bone
    (2022-06-13) Fernández López, Lucía; Mancini, Rosanna; Rottolo, Maria Concetta; Navarro Zaragoza, Javier; Hernández del Rincón, Juan Pedro; Falcón Romero, María; Farmacología
    Carbamazepine is the main option used as a preventive medication to treat bipolar disorder when there is no response to lithium. Carbamazepine toxicity is defined as serum levels greater than 12 μg/mL, with severe toxicity occurring over 40 μg/mL, reduced to 30 μg/mL when combined with pharmacological treatment, i.e., benzodiazepines or antidepressants. For these reasons, it is necessary to find a validated tool to determine carbamazepine levels in an autopsy to rule out suicide or to know if the death was a consequence of an adverse drug reaction (ADR), especially when only bones can be accessed. We have validated a tool to detect and quantify drug concentration in bone. Our results showed a peak for carbamazepine at minute 12 and a mass fragment of 193 m/z. This case study is the first time in the literature that carbamazepine has been detected and quantified in bone. These results demonstrate that carbamazepine can be detected in bone tissue from forensic cases, but almost more importantly, that the method proposed is valid, reliable, and trustworthy.
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    Del trastorno del diálogo tónico a la inestabilidad psicomotriz: Taxonomía diagnóstica
    (Universidad de Murcia, Servicio de Publicaciones, 2022) Boscaini, Franco; Cachón Zagalaz, Javier; Díaz Suárez, Arturo
    El objetivo de este trabajo es profundizar en la relación entre hiperactividad y diálogo tónico al considerar el cuerpo como elemento común de comunicación, aunque sus manifestaciones psicomotoras y significados sean diferentesdurante el desarrollo.Mientras que el diálogo tónico es vital para que el niño establezca el vínculo de apego, la inestabilidad psicomotora es la expresión de una dificultad para relacionarse con la realidad. La complejidad y variabilidad de los cuadros clínicos dificulta su ubicación en las clasificaciones diagnósticas internacionales. La investigación luego confirma las consecuencias del trastorno del diálogo tónico, constituyendo un modelo sobre el que se organizarán los comportamientos futuros.Los autores plantean la hipótesis de que cada etapa del diálogo tónico, si se vive con dificultad, constituye una matriz sobre la que se estructurarán diversas expresiones de inestabilidad. La intención es recopilar material teórico-clínico para posteriormente realizar una clasificación diagnóstica de la inestabilidad psicomotora.
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    Multichromatic TTF staining characterizes cartilage matrix in osteoarthritis and bone development
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Feng, Yu; Cai, Zhe; Cheung, Wing ki; Yang, Kedi; Xu, Lei; Weijia Lu, William; Yang, Haibo; Chiu, Kwong-Yuen
    Various histological staining methods have been explored to detect the joint lesions in osteoarthritis (OA), but these histological stains cannot comprehensively present the comparatively complex structures of articular cartilage in knee OA. In addition, no integrated histological staining method can be used to evaluate efficiently both the subzone region and matrix composition in cartilage containing tissues. Therefore, in this study, a novel multichromatic staining method termed TTF staining, using Toluidine Blue (T), Tartrazine (T) and Fast Green (F) sequential combined staining for histological analysis, has been exploited to characterize the changes of matrix components and contents in cartilage during OA and in the bone development. This specific TTF staining profile can be used to differentiate the major compartments of knee joint region, including the synovium, meniscus, multiple subzones of cartilage and subchondral bone. An anterior cruciate ligament transection induced OA model in rat has been established to profoundly present the alterations of glycosaminoglycans in cartilage degeneration by TTF staining profile. The changes of TTF staining profile in the chondrification and ossification centers of the postnatal rat knee joint indicate the developmental features of cartilage matrix during the growth of bone. In summary, we have developed an effective histological staining method that enables us to identify the subzones of cartilage in detail and to define the matrix features of bone development. Therefore, finally using this new TTF staining method may help us to exploit a histopathological grading system to assess cartilage lesions in clinical disease.
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    El sentido de las cuestiones previas en el Proyecto Roma
    (Universidad de Zaragoza, Asociación Universitaria de Formación del Profesorado (AUFOP), 2018) González Martínez, Ana María
    En este artículo muestro cómo descubrí el Proyecto Roma y cómo ha cambiado mi visión de la educación desde aquel instante. También explico, desde mi experiencia, cómo he llevado a cabo las cuestiones previas en mi clase de una forma muy sencilla y práctica, intentando así que sea accesible no solo a profesionales de la educación sino también a cualquier familia o incluso estudiante.
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    Unveiling the smallest – systematics, classification and a new subfamily of featherwing beetles based on larval morphology (Coleoptera: Ptiliidae).
    (CSIRO Publishing, 2019) Sörensson, M.; Delgado, Juan A.; Zoología y Antropología Física