Browsing by Subject "Emperipolesis"

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    Glycosaminoglycan-sac formation in vitro. Interactions between normal and malignant cells
    (Murcia : F. Hernández, 1994) Logothetou-Rella, H.
    The interaction of monolayer normal human or normal rat cells with suspension Walker rat tumor cells was demonstrated cytologically, during a cocultivation period of thirty days. At ten days, Walker rat tumor cells were interiorized in the cytoplasm of the normal monolayer host cells. At twenty days, degeneration of the interiorized tumor cells followed by mucification led to glycosaminoglycan-sac formation. At thirty days, tumor nodules and protease (a,- chymotrypsin)- bound glycosaminoglycan(s) extracellular matrix, transversing the culture in membranous rivulets, were formed. The overall interaction resulted in the death of the interiorized tumour cell and survival of the normal host cells containing a glycosaminoglycansac. The use of bladder tumor cell imprints showed that cell interiorization, degeneration and extracellular matrix formation identical to that of the CO-cultures, also occur in vivo. Cell interiorization within malignant cell cultures led to syncytia formation and survival of both host and interiorized cells. Identical glycosaminoglycan extracellular matrix to that of the CO-cultures and syncytia was also observed in smears of EBV-producing (P3HR-l) cell cultures. The observations are discussed in terms of invasion, emperipolesis, cannibalism, phagocytosis and extracellular matrix. It is concluded that a glycosaminoglycan-sac is produced by the interiorization of a tumor cell into a normal cell while interiorization of a tumor cell into another tumor cell resulted in syncytia formation.
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    High-grade renal cell carcinoma with emperipolesis: Clinicopathological, immunohistochemical and molecular-genetic analysis of 14 cases
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2018) Rotterova, Pavla; Martinek, Petr; Alaghehbandan, Reza; Prochazkova, Kristyna; Damjanov, Iván; Rogala, Joanna; Suster, Saul; Perez Montiel, Delia; Alvarado Cabrero, Isabel; Sperga, Maris; Švajdler, Marián; Michalova, Kvetoslava; Pivovarcikova, Kristyna; Daum, Ondrej; Hora, Milan; Dusek, Martin; Ondic, Ondrej; Stehlikova, Adela; Michal, Michal; Hes, Ondrej
    Emperipolesis has recently been described as a constant feature of “biphasic squamoid” papillary renal cell carcinoma (BPRCC). We also noticed this in some high-grade (HG) RCC, which promoted the present study to estimate the incidence of emperipolesis in RCCs and to describe them in further detail. 14 cases of HGRCC showing emperipolesis were retrieved from our registry. Microscopic examination of filed slides was supplemented with immunohistochemical and molecular-genetic analyses using paraffin embedded tissue. 12 of 14 patients were males with a mean age of 58.6 years (range 41-72 years). Tumor size ranged from 6-16.5 cm (mean of 8.8 cm). Follow up data were available for 8/14 patients (range 0.5-10 years). Metastases were documented in 6 cases. All tumors showed solid-alveolar growth patterns with focal pseudopapillary features, and were composed of large cells with bizarre nuclei and eosinophilic rhabdoid-like cytoplasm. Emperipolesis was a constant and prominent feature in large bizarre cells. All cases were positive for OSCAR, CANH 9, vimentin, cyclin D1, INI-1, and myoD1, while negative for melanocytic markers, CK 7, myoglobin, cathepsin K, and TFE3. VHL gene abnormalities were found in 6/9 analyzable cases, of which 2 demonstrated polysomy of chromosomes 7, 17. Emperipolesis is a rare histomorphologic feature which can be seen not only in BPRCCs but also in highgrade CCRCCs. All RCC cases with prominent emperipolesis fulfilled both morphologic and immunohistochemical diagnostic criteria of high-grade CCRCC. The majority of patients with available follow up information developed metastases.