Publication: Glycosaminoglycan-sac formation in vitro. Interactions between normal and malignant cells
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Date
1994
Authors
Logothetou-Rella, H.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The interaction of monolayer normal human
or normal rat cells with suspension Walker rat tumor
cells was demonstrated cytologically, during a cocultivation
period of thirty days. At ten days, Walker rat
tumor cells were interiorized in the cytoplasm of
the normal monolayer host cells. At twenty days,
degeneration of the interiorized tumor cells followed by
mucification led to glycosaminoglycan-sac formation.
At thirty days, tumor nodules and protease (a,-
chymotrypsin)- bound glycosaminoglycan(s) extracellular
matrix, transversing the culture in membranous
rivulets, were formed. The overall interaction resulted in
the death of the interiorized tumour cell and survival of
the normal host cells containing a glycosaminoglycansac.
The use of bladder tumor cell imprints showed that
cell interiorization, degeneration and extracellular matrix
formation identical to that of the CO-cultures, also occur
in vivo. Cell interiorization within malignant cell
cultures led to syncytia formation and survival of both
host and interiorized cells. Identical glycosaminoglycan
extracellular matrix to that of the CO-cultures and
syncytia was also observed in smears of EBV-producing
(P3HR-l) cell cultures. The observations are discussed
in terms of invasion, emperipolesis, cannibalism,
phagocytosis and extracellular matrix. It is concluded
that a glycosaminoglycan-sac is produced by the
interiorization of a tumor cell into a normal cell while
interiorization of a tumor cell into another tumor cell
resulted in syncytia formation.
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