Browsing by Subject "DNA"

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    Clinico-pathological correlations in meningiomas, a DNA and immunohistochemical study
    (Murcia : F. Hernández, 1993) Cruz-Sánchez, F. F.; Miquel, R.; Rossi, M.L.; Figols, J.; Palacín, A.; Cardesa, Antonio
    We have studied 41 meningiomas classified histologically as benjgn, atypical or anaplastic. There were 26 females and 15 males and the mean age was 53 years. 36 tumours were supratentorial, 4 infratentorial and one spinal. Flow cytometry was performed on paraffin-embedded tissue using a selective staining technique for DNA. The ploidy index of DNA and percentage of cells in the S and G2/M phases were calculated. Results were correlated with clinical, histological and immunohistological data. 16/41 tumours . were found to be diploid, 17/41 aneuploid and 8/41 could not be analysed. Significant correlations were found between aneuploid tumours and some qualitative features such as recurrence, pleomorphism, high cellular density, mitotic activity and brain and soft tissue infiltration. A high proliferative index appeared to be associated with clinical aggressiveness. No particular correlation between the expression of cytokeratin and epithelial membrane antigen markers and flow cytometry was found. Our results suggest that DNA flow cytometry in meningiomas may be of value in predicting the behaviour of these neoplasms and confirm that epithelial pattern in meningiomas is not linked to increased anaplasia or poor prognosis.
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    DNA-Ploidy, morphometric-stereological and P-Glycoprotein study of superficial bladder carcinomas
    (S. Karger AG, 1992) Sánchez Fernández de Sevilla, M. C.; Gil Salom, M.; Pérez Bacete, M.; Morell Cuadreny, L.; Martínez Díaz, F.; Iborra Juan, I.; Fenollosa Entrena, B.; Llombart Bosch, A.; Oftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
    We carried out a DNA-ploidy, morphometri􀀜stereolcgic and P-glycoprotein study on 40 newly diagnosed superficial bladder cancer patients (G l-G2), cor­relating the results with histological grade and clinical outcome. Variations in the number of patients who present recurrences, progression or remain tumor-free during the whole follow-up period (at least 5 years) were not signif­icant when related to nuclear size, proliferative diploid index, presl!nce of aneuploidy and expression of P-glycoprotein. lt is striking how the majority of disease-free subjects showed a proliferative diploid index higher than 10%. Moreoyer .. 3 of them presented a11 aneuploid cell population. In our study, only histological grade showed a significant discriminatory level in terms of progression versus no progression in patients with superficial bladder cancer.
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    Nonsteroidal anti-inflammatory drugs and oxidative stress in cancer cells
    (Murcia : F. Hernández, 2007) Adachi, M.; Sakamoto, H.; Kawamura, R.; Wang, W.; Imai, K.; Shinomura, Y.
    Nonsteroidal antiinflammatory drugs (NSAIDs) induce apoptosis in a variety of cancer cells, including those of colon, prostate, breast and leukemia. In addition, the classical NSAIDs sulindac and aspirin are promising chemopreventive agents against colon cancer. NSAIDs inhibit cyclooxygenases (COX) preventing the formation of prostaglandins, prostacyclin and thromboxane. NSAIDs also exert other biological effects, including generation of reactive oxygen species (ROS) and inhibition of NF-kB-mediated signals. Despite many suggested mechanisms for their anticancer effects, it remains uncertain how they induce cell cycle arrest and apoptosis in cancer cells. Furthermore, there is little information on the selectivity of NSAIDs-mediated anticancer effects, although this is one of the most important issues in cancer therapy. Increased understanding of the biological basis for the anticancer activity of NSAIDs and their selectivity is essential for future therapeutic advances. In this paper, we propose that increased ROS generation is one of the key mechanisms for NSAIDs-mediated anticancer effects on various cancer cells.